Total Protein Extraction Kit – Invent Biotechnologies Inc.

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Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)

Read more literature references here

Catalog number: SD-001/SN-002

  • $365.00
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Manual & ProtocolMaterial Safety Data Sheets (MSDS)

The Minute™ kit is a next-generation, spin column–based system designed for rapid and efficient total protein extraction from animal cultured cells and tissues. Unlike conventional solution-based methods, such as RIPA buffer, which often result in protein loss and altered profiles, this patented technology preserves protein integrity and yields reproducible results.

With enhanced lysis buffers and a streamlined protocol, proteins can be extracted in just 1–5 minutes at concentrations ranging from 1–8 mg/ml. The system supports both denaturing and native conditions, allowing users to choose based on downstream applications. Minimal extraction volume (as low as 20 µl) makes it ideal when starting material is limited.

This kit offers one of the fastest protocols available for total protein preparation and can be readily adapted for high-throughput workflows. The extracted proteins are also suitable as starting material for small-scale purification using column chromatography.

-RIPA Buffer - The Potential Troubles

-Pitfalls of Protein Extraction by RIPA

-A Technical Breakthrough for Protein Sample Preparation

Selected references related to protein loss by RIPA buffer:

  1. Bai, B., and Laiho, M. (2012) Proteomics. 12:3044-3048
  2. Mukhopadhyay, C. et al. (2016) PNAS 5:8228-8237
  3. Li, Q. (2016) Biotechniques. 61:327
  4. Ngoka, L. CM. (2008) Proteome Science. 6:30

    Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver.    Kit includes:

    Items

    Quantity

    Denaturing Cell Lysis Buffer

    25 ml

    Native Cell Lysis Buffer

    25 ml

    Protein Extraction Filter Cartridges

    50 units

    Collection Tubes with Caps

    50 units

    Plastic Rods

    2 units




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