Minute™ Total Protein Extraction Kit (for Animal Cultured Cells and Tissues (50 Preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues is the most advanced tool for rapid protein extraction without altered protein profile. More and more evidence has shown that the most commonly used RIPA buffer can cause unpredictable protein loss, resulting in questionable data interpretation. This problem is fully resolved by the patented spin-column-based technologies, which when used with stronger lysis buffers can effectively extract proteins from tough tissues such as skin, blood vessels, muscle, etc. When starting material is a limiting factor, the use of the proprietary protein extraction filter cartridges can be a helpful feature. This allows the extraction volume to be as low as 20 µl. This kit provides both denaturing and native cell lysis, so users can select according to specific applications. Total proteins can be extracted from cultured cells/tissues in 1-8 minutes with high yield (2-8 mg/ml).

Selected references related to protein loss by RIPA buffer:

1. Bai, B., and Laiho, M. (2012) Proteomics. 12:3044-3048
2. Mukhopadhyay, C. et al. (2016) PNAS 5:8228-8237
3. Li, Q. (2016) Biotechniques. 61:327
4. Ngoka, L. CM. (2008) Proteome Science. 6:30

Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver. 

Kit includes:

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12. Bashir, K. M. I., & Cho, M. G. (2016). The Effect of Kanamycin and Tetracycline on Growth and Photosynthetic Activity of Two Chlorophyte Algae. BioMed Research International, 2016.
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16. Kampert, T., Misra, S. K., Srivastava, I., Tripathi, I., & Pan, D. (2017). Phenotypically Screened Carbon Nanoparticles for Enhanced Combinatorial Therapy in Triple Negative Breast Cancer. Cellular and Molecular Bioengineering, 1-16.
17. Zhang, C., Hong, F. F., Wang, C. C., Li, L., Chen, J. L., Liu, F., ... & Wang, J. F. (2017). TRIB3 inhibits proliferation and promotes osteogenesis in hBMSCs by regulating the ERK1/2 signaling pathway. Scientific Reports, 7.
18. Inoue, T., Ninuma, S., Hayashi, M., Okuda, A., Asaka, T., & Maejima, H. (2017). Effects of long-term exercise and low-level inhibition of GABAergic synapses on motor control and the expression of BDNF in the motor related cortex. Neurological Research, 1-8.
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21. Misra, S. K., De, A., & Pan, D. (2017). Targeted Delivery of STAT-3 Modulator to Breast Cancer Stem Like Cells Down-regulates a Series of Stem-ness Genes. Molecular Cancer Therapeutics, molcanther-0070.
22. Liao, P., Yang, D., Liu, D., & Zheng, Y. (2017). GLP-1 and Ghrelin Attenuate High Glucose/High Lipid-Induced Apoptosis and Senescence of Human Microvascular Endothelial Cells. Cellular Physiology and Biochemistry, 44(5), 1842-1855.
23. Guo, Q., Wu, D., Fan, C., Peng, S., Guan, H., Shan, Z., & Teng, W. (2018). Iodine excess did not affect the global DNA methylation status and DNA methyltransferase expression in T and B lymphocytes from NOD. H-2 h4 and Kunming mice. International Immunopharmacology, 55, 151-157.
24. Misra, S. K., De, A., & Pan, D. (2018). Targeted Delivery of STAT-3 Modulator to Breast Cancer Stem-Like Cells Downregulates a Series of Stemness Genes. Molecular cancer therapeutics, 17(1), 119-129.
25. Xue, M., Ji, X., Liang, H., Liu, Y., Wang, B., Sun, L., & Li, W. (2018). The effect of fucoidan on intestinal flora and intestinal barrier function in rats bearing breast cancer. Food & Function.
26. Su, X., Tang, W., Luan, Z., Yang, Y., Wang, Z., Zhang, Y., ... & Yuan, H. Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury. Experimental and Therapeutic Medicine.
27. Zhao, J., Wu, X., Wu, D., Yu, Y., Yu, Y., Wang, Y., ... & Yao, K. (2018). Embryonic Surface Ectoderm-specific Mitofusin 2 Conditional Knockout Induces Congenital Cataracts in Mice. Scientific Reports, 8(1), 1522.
28. Guan, X., Lu, Y., Wang, G., Gibson, P., Chen, F., Fang, K., & Yuan, Y. (2018). The Role of Regulatory T Cell in Nontypeable Haemophilus influenzae-Induced Acute Exacerbation of Chronic Obstructive Pulmonary Disease. Mediators of Inflammation, 2018.
29. Yin, X., Feng, L., Ma, D., Yin, P., Wang, X., Hou, S., & Feng, J. (2018). Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone. Journal of Neuroinflammation, 15(1), 97.
30. Lu, J., Fang, K., Wang, S., Xiong, L., Zhang, C., Liu, Z. & Wang, F. (2018). Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation, 2018.
31. Li, X., Yang, L., Li, D., Zhu, Y., Wang, Q., & Li, W. (2018). Pathogen-specific Binding soluble Down syndrome cell adhesion Molecule (Dscam) regulates Phagocytosis via Membrane-Bound Dscam in crab. Frontiers in immunology, 9, 801.
32. Chen, Y., Li, M., Zhang, Y., Di, M., Chen, W., Liu, X., & Zhang, M. (2018). Traditional Chinese medication Tongxinluo attenuates apoptosis in ox-LDL-stimulated macrophages by enhancing Beclin-1-induced autophagy. Biochemical and biophysical research communications.
33. Bashir, K. M. I., Kim, M. S., Stahl, U., & Cho, M. G. (2018). Agrobacterium-mediated genetic transformation of Dictyosphaerium pulchellum for the expression of erythropoietin. Journal of Applied Phycology, 1-16.
34. Wang, C., Chen, W. J., Wu, Y. F., You, P., Zheng, S. Y., Liu, C. C., ... & Borjigin, U. (2018). The extent of liver injury determines hepatocyte fate toward senescence or cancer. Cell death & disease, 9(5), 575. Guo, Q., Wu, Y., Hou, Y., Liu, Y., Liu, T., Zhang, H & Teng, W. (2018). Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Frontiers in immunology, 9.
35. Guo, Q., Wu, Y., Hou, Y., Liu, Y., Liu, T., Zhang, H., & Teng, W. (2018). Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Frontiers in immunology, 9.
36. Maejima, H., Ninuma, S., Okuda, A., Inoue, T., & Hayashi, M. (2018). Exercise and low-level GABAA receptor inhibition modulate locomotor activity and the expression of BDNF accompanied by changes in epigenetic regulation in the hippocampus. Neuroscience Letters.
37. Jiang, Q., Liu, H., Wang, S., Wang, J., Tang, Y., He, Z., & Liang, C. (2018). Circadian locomotor output cycles kaput accelerates atherosclerotic plaque formation by upregulating plasminogen activator inhibitor-1 expression. Acta Biochimica et Biophysica Sinica
38. Zhang, J., Cai, H., Sun, L., Zhan, P., Chen, M., Zhang, F., ... & Wan, J. (2018). LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients. Journal of Experimental & Clinical Cancer Research, 37(1), 225.
39. Zhang, M., Fu, Z., Chen, J., Zhu, B., Cheng, Y., & Fu, L. (2018). Low level expression of the Mitochondrial Antiviral Signaling protein (MAVS) associated with long-term nonprogression in SIV-infected rhesus macaques. Virology Journal, 15(1), 159.
40. Liang, Y., Zhang, T., Shi, M., Zhang, S., Guo, Y., Gao, J., & Yang, X. Low expression of NCOA5 predicts poor prognosis in human cervical cancer and promotes proliferation, migration, and invasion of cervical cancer cell lines by regulating notch3 signaling pathway. Journal of Cellular Biochemistry.
41. Zhang, X. M., Huang, Y., Cong, X., Qu, L. H., Zhang, K., Wu, L. L., ... & Yu, G. Y. Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term. Journal of Cellular Physiology.
42. Ding, M., Zhang, H., Liu, L., & Liang, R. Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells. Oncology Letters.
43. Fu, Y., Dong, J., Wang, J., You, M., Wei, L., Fu, H., ... & Chen, J. (2018). Developmental Exposure to Di-(2-ethylhexyl) Phthalate Induces Cerebellar Granule Cell Apoptosis via the PI3K/AKT Signaling Pathway. Experimental Neurobiology, 27(6), 472-488.
44. Wang, Z., Fang, K., Wang, G., Guan, X., Pang, Z., Guo, Y., ... & Wang, F. (2019). Protective effect of amygdalin on epithelial–mesenchymal transformation in experimental chronic obstructive pulmonary disease mice. Phytotherapy Research.
45. Coskun, E., Tuna, G., Jaruga, P., Tona, A., Erdem, O., & Dizdaroglu, M. (2019). Identification and quantification of DNA repair protein poly (ADP ribose) polymerase 1 (PARP1) in human tissues and cultured cells by liquid chromatography/isotope-dilution tandem mass spectrometry. DNA Repair.