Minute™ High-Efficiency Protein Precipitation Kit (30 ml)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Protein precipitation is an obvious choice for concentrating proteins and removing interfering substances found in protein samples, such as salts, lipids, and other components that may interfere with downstream applications. One of the most commonly used methods is trichloroacetic acid (TCA) precipitation, a relatively simple and effective method. Protein precipitated by the TAC method is usually denatured, and in many cases, the solubility of protein is reduced. A major disadvantage of the traditional TCA/acetone precipitation method is its low efficiency for samples of low protein concentration. Samples with higher protein concentration precipitate much more effectively than that with lower protein concentration. To overcome the shortcomings, we have developed this high-efficiency protein precipitation kit, which is a modification of the traditional TCA method. It is simple, rapid, and highly effective. Proteins at low concentrations can be effectively precipitated and concentrated in less than 30 min, and the precipitated protein can be re-dissolved readily in buffers containing detergent(s).

Kit includes: