Protein Extraction Kit for Hair and Nails (20 Preps)

Manual & Protocol | MSDS

Proteins account for about 80% of keratinized animal tissues such as hair, nail, horn and wool. These tissues contain mainly hard keratins made of several distinct types of protein with a molecular weight ranging from 10 to 135 kDa. The extraction buffer usually contains a high concentration of urea, thiourea, guanidine hydrochloride or a combination of these strong denaturants. Protein extraction with high concentration denaturants is relatively effective, but because of larger extraction buffer/tissue ratio, the concentration of extracted protein is usually low. Extracted proteins need to be concentrated prior to further analysis. The presence of high-concentration denaturants may also interfere with downstream application. HD-021 is designed to effectively extract proteins from keratinized animal tissues without using strong denaturants. The extracted protein can be directly loaded onto SDS-PAGE without concentration. The extraction buffer of this kit contains 0.5% SDS and other chemicals. Extracted protein concentration is 1-2 mg/ml.

Kit includes:

References (7)

1. Lee, S. H., Baldina, E., Lee, E., & Youm, Y. (2021). Social connectedness and hair cortisol in community-dwelling older adults. Comprehensive Psychoneuroendocrinology, 100053.
2. Marsden, A. J., Riley, D. R., Birkett, S., Rodriguez-Barucg, Q., Guinn, B. A., Carroll, S., ... & Beltran-Alvarez, P. (2021). Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers. Amino Acids, 1-10.
3. Kim, W. J., Park, K. M., Park, J. T., Seo, E., An, S. K., Park, H. Y., & Lee, E. (2021). Sex-specific association of hair cortisol concentration with stress-related psychological factors in healthy young adults. Biology of Sex Differences, 12(1), 1-7.
4. Kim, W. J., Park, K. M., Park, J. T., Seo, E., Bang, M., An, S. K., ... & Lee, E. (2021). Effect of Childhood Trauma on the Association Between Stress-Related Psychological Factors and Hair Cortisol Level in Young Adults. Psychiatry Investigation, 18(11), 1131.
5. Lee, S., Park, J. T., Bang, M., An, S. K., Namkoong, K., Park, H. Y., & Lee, E. (2022). Theory of mind and hair cortisol in healthy young adults: the moderating effects of childhood trauma. European Journal of Psychotraumatology, 13(2), 2116826.
6. Fuse, N., Morita, S., & Matsue, Y. (2023). Age‐related hair denaturation related to protein carbonyls. International Journal of Cosmetic Science.
7. Fuse, N., Morita, S., & Matsue, Y. (2023). Age‐related hair denaturation related to protein carbonyls. International Journal of Cosmetic Science.
8. Li, Z., Kong, W., Park, H. Y., Koo, S. J., Bang, M., Park, J. T., ... & An, S. K. (2024). Association of hair cortisol concentration with brain‐derived neurotrophic factor gene methylation: The role of sex as a moderator. Stress and Health, e3401.