Minute™ Histone/DNA Binding Protein Extraction Kit (50 Preps)

Manual & Protocol | MSDS

Histones and other DNA-binding proteins play an important role in chromosome organization, gene regulation, and posttranslational modification. Core histone components can be covalently modified through methylation, acetylation, phosphorylation, and sumoylation. These modifications are essential for gene expression, regulation, DNA repair, and chromosome condensation. HP-014 is designed to rapidly extract histones and other DNA-binding proteins from cultured cells and cells isolated from animal tissues. Unlike many commercial histone extraction kits that use acid or high-salt extraction methods, our kit extracts histones through a completely different mechanism. This kit is equally efficient in extracting histones and other DNA-binding proteins that can be used for internal controls for histone modification analysis. In comparison, the acid extraction protocol selectively extracts basic proteins, and a good internal control is usually not possible. This kit extracts histones from 0.5 to 5 million cells in less than 10 minutes with high protein yield (1-2.5 mg/ml), making it the fastest and most robust histone extraction kit on the market.

Kit includes: