Minute™ Endosome Isolation and Cell Fractionation Kit (20 Preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Early endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues.

Kit includes:

1. Xu et al., (2018) Phosphorylation-Mediated IFN-gR2 MembraneTranslocation Is Required to Activate Macrophage Innate Response.  Cell 175, 1–16.
2. Drelich, A., Judy, B., He, X., Chang, Q., Yu, S., Li, X., ... & Ksiazek, T. (2018). Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells. Viruses, 10(10), 563.
3. Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.
4. Thapa, N., Chen, M., Horn, H. T., Choi, S., Wen, T., & Anderson, R. A. (2020). Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4. Nature Cell Biology, 22(11), 1357-1370.
5. Liang, X. H., Nichols, J. G., Hsu, C. W., & Crooke, S. T. (2021). Hsc70 Facilitates Mannose-6-Phosphate Receptor-Mediated Intracellular Trafficking and Enhances Endosomal Release of Phosphorothioate-Modified Antisense Oligonucleotides. nucleic acid therapeutics.
6. Jin, J., Kim, C., Xia, Q., Gould, T. M., Cao, W., Zhang, H., ... & Goronzy, J. J. (2021). Activation of mTORC1 at late endosomes misdirects T cell fate decision in older individuals. Science Immunology, 6(60).

7. Gong, B., Guo, Y., Ding, S.et al.A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission.Nat Cell Biol (2021). https://doi.org/10.1038/s41556-021-00704-y

8. Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.
9. Ren, X., Zhang, S., Yang, Y., Song, A., Liang, F., Zhang, Y., ... & Xie, Z. (2022). Ketamine induces delirium-like behavior and interferes with endosomal tau trafficking.
10. Wang, C. K., Nelepcu, I., Hui, D., Oo, H. Z., Truong, S., Zhao, S., ... & Daugaard, M. (2022). Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cells. Scientific Reports, 12(1), 1-13.
11. Zhu, Y., Wu, Z., Yan, W., Shao, F., Ke, B., Jiang, X., ... & Sun, Y. (2022). Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis. EMBO molecular medicine, 14(3), e14455.
12. Ren, W., Lou, H., Ren, X., Wen, G., Wu, X., Xia, X., ... & Wu, X. (2022). Ketamine promotes the amyloidogenic pathway by regulating endosomal pH. Toxicology, 471, 153163.
13. Ding et al. (2022). Intracellular compelement C5a/C5aR1 stabilizes b-catenin to promote colorectal tumorigenesis. Cell Reports 39, 110851.
14. Lee, J., Mohammad, N., Lu, Y., Kang, K., Han, K., & Brantly, M. (2022). Alu RNA induces NLRP3 expression through TLR7 activation in α-1-antitrypsin–deficient macrophages. JCI Insight, 7(12).
15.  Basukala, O., Trejo-Cerro, O., Myers, M. P., Pim, D., Massimi, P., Thomas, M., ... & Banks, L. (2022). HPV-16 E7 Interacts with the Endocytic Machinery via the AP2 Adaptor μ2 Subunit. Mbio, e02302-22.
16. Zhu, F., Yu, D., Qin, X., Qian, Y., Ma, J., Li, W., ... & Xia, P. (2023). The neuropeptide CGRP enters the macrophage cytosol to suppress the NLRP3 inflammasome during pulmonary infection. Cellular & Molecular Immunology, 1-13.
17. Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
18. Park, S., Park, D., Han, S., Chung, G. E., Soh, S., Ka, H. I., ... & Yang, Y. (2023). LY6K depletion modulates TGF‐β and EGF signaling. Cancer Medicine.
19. Panfili, E., Mondanelli, G., Orabona, C., Gargaro, M., Volpi, C., Belladonna, M. L., ... & Pallotta, M. T. (2023). The catalytic inhibitor epacadostat can affect the non-enzymatic function of IDO1. Frontiers in Immunology, 14.
20. Pan, C. C., Maeso-Díaz, R., Lewis, T. R., Xiang, K., Tan, L., Liang, Y., ... & Wang, X. F. (2023). Antagonizing the irreversible thrombomodulin-initiated proteolytic signaling alleviates age-related liver fibrosis via senescent cell killing. Cell Research, 1-17.
21.  Zhang, Q., Duan, Q., Gao, Y., He, P., Huang, R., Huang, H., ... & Wang, L. Cerebral Microvascular Injury Induced by Lag3‐Dependent α‐Synuclein Fibril Endocytosis Exacerbates Cognitive Impairment in a Mouse Model of α‐Synucleinopathies. Advanced Science, 2301903.

22. Yu, D. M., Zhao, J., Lee, E. E., Kim, D., Mahapatra, R., Rose, E. K., ... & Wang, R. C. (2023). GLUT3 promotes macrophage signaling and function via RAS-mediated endocytosis in atopic dermatitis and wound healing. The Journal of clinical investigation, 133(21).

23. Loss of Wei, Y., Chen, Q., Chen, J., Zhou, C., Geng, S., Shi, D., ... & Jiang, J. (2023). Loss of α-1, 2-mannosidase MAN1C1 promotes tumorigenesis of intrahepatic cholangiocarcinoma through enhancing CD133-FIP200 interaction. Cell Reports, 42(12), 113588.
24. Thapa, N., Chen, M., Cryns, V. L., & Anderson, R. A. (2024). Enhanced PI3K/Akt Signaling in Response to p85α Loss is Regulated by Endosomal PI3Kα and PI3P. bioRxiv, 2024-01.
25. Liao, Z., Ji, W., Yang, C., & Su, J. (2024). TLR5M and TLR5S Synergistically Sense Flagellin in Early Endosome in Lamprey Petromyzon marinus, Switched by the N-Glycosylation Site N239. The Journal of Immunology.
26. Jiang Y, Chen X, Ye X, Wen C, Xu T, Yu C, Ning W, Wang G, Xiang X, Liu X, Wang Y, Chen Y, Liu X, Shi C, Liu C, Yuan Q, Chen Y, Zhang T, Luo W, Xia N. A Dual-domain Engineered Antibody for Efficient HBV Suppression and Immune Responses Restoration. Adv Sci (Weinh). 2024 Feb 11:e2305316. doi: 10.1002/advs.202305316. Epub ahead of print. PMID: 38342604.
27. Li, B., Wang, S., Cai, Z., Li, J., Li, J., Kerman, B. E., ... & Yassine, H. ABCA1 Deficiency Impairs Endosomal-Lysosomal Trafficking of Glutamate Receptors in the Brain. Available at SSRN 4733242.
28. Thapa, N., Chen, M., Cryns, V. L., & Anderson, R. (2024). A p85 isoform switch enhances PI3K activation on endosomes by a MAP4-and PI3P-dependent mechanism. Cell Reports, 43(5), 114119.
29. Liu, J., Heddleston, J., Perkins, D. R., Chen, J. J. H., Ghanbarpour, A., Smith, B. W., ... & Afshar, S. (2024). Discovery of a New Class of Cell-Penetrating Peptides by Novel Phage Display Platform. Researchsqare.
30. Giamundo, G., Intartaglia, D., Del Prete, E., Polishchuk, E., Andreone, F., Ognibene, M., ... & Conte, I. (2024). Ezrin defines TSC1 activation at endosomal compartments through EGFR-AKT signaling. bioRxiv, 2024-05. 
31. Toh, S. Y., Leong, H. S., Chong, F. T., Rodrigues-Junior, D. M., Ren, M. J., Kwang, X. L., ... & Iyer, N. G. (2024). Therapeutic application of extracellular vesicular EGFR isoform D as a co-drug to target squamous cell cancers with tyrosine kinase inhibitors. Developmental Cell.
32. Panfili, E., Rezzi, S. J., Adamo, A., Mazzoletti, D., Massarotti, A., Miggiano, R., ... & Pallotta, M. T. (2024). Identification of a Compound Inhibiting Both the Enzymatic and Nonenzymatic Functions of Indoleamine 2, 3-Dioxygenase 1. ACS Pharmacology & Translational Science.
33. Lin, Y., Zheng, H., Jia, L., Luo, Y., Zhang, D., An, M., ... & Chen, C. (2024). Integrin α6‐containing extracellular vesicles promote lymphatic remodelling for pre‐metastatic niche formation in lymph nodes via interplay with CD151. Journal of Extracellular Vesicles, 13(10), e12518.