Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps)

Manual & Protocol | MSDS

Lipid rafts are small membrane domains containing a high level of cholesterol and sphingolipids. Lipid rafts have been found in the plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. Lipid rafts are implicated in numerous cellular processes such as signal transduction, membrane trafficking, and protein sorting.  Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na⁺/K⁺ ATPase on PM. Traditional methods for lipid raft isolation involve isolation of detergent-resistant membrane subdomain from total membranous structures, which does not distinguish plasma membrane-derived and/or organelle-derived lipid rafts. Using the patented spin-column-based technologies, we have developed this kit specifically for the isolation of plasma membrane-derived lipid rafts. Larger plasma membrane vesicles are first isolated and treated with a non-ionic detergent containing buffer followed by isolation of detergent-resistant fraction by flotation centrifugation using a tabletop microcentrifuge. Highly enriched plasma membrane-derived lipid rafts can be obtained in about 1 hour without using a traditional homogenizer and ultracentrifugation.

*For total lipid raft isolation, please refer to Minute^TM Total Lipid Raft Isolation Kit under Cat # LR-039.

Kit Components:

 References (12)

1. Bu, Y., Teng, Q., Feng, D., Sun, L., Xue, J., & Zhang, G. (2021). YLMY Tyrosine Residue within the Cytoplasmic Tail of Newcastle Disease Virus Fusion Protein Regulates Its Surface Expression to Modulate Viral Budding and Pathogenicity. Microbiology Spectrum, 9(3), e02173-21.
2. Rashkovan, M., Albero, R., Gianni, F., Perez-Duran, P., Miller, H. I., Mackey, A. L., ... & Ferrando, A. A. (2021). Intracellular cholesterol pools regulate oncogenic signaling and epigenetic circuitries in Early T-cell Precursor Acute Lymphoblastic Leukemia. Cancer discovery.
3. Jiang, C., Lin, Y., Shan, H., Xia, W., Pan, C., Wang, N., ... & Yu, X. (2022). miR-146a Protects against Staphylococcus aureus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis. ACS Infectious Diseases.
4. Fiore, D., Proto, M. C., Franceschelli, S., Pascale, M., Bifulco, M., & Gazzerro, P. (2022). In Vitro Evidence of Statins’ Protective Role against COVID-19 Hallmarks. Biomedicines, 10(9), 2123.
5. Lim, J. H., Ahmad, K., Chun, H. J., Hwang, Y. C., Qadri, A. F., Ali, S., ... & Lee, E. J. (2022). IgLON4 Regulates Myogenesis via Promoting Cell Adhesion and Maintaining Myotube Orientation. Cells, 11(20), 3265.
6. Sha, Y. L., Liu, Y., Yang, J. X., Wang, Y. Y., Gong, B. C., Jin, Y., ... & Zhao, Q. (2022). B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma. Journal of Experimental & Clinical Cancer Research, 41(1), 1-21.
7. Gao, S. S., Shi, R., Sun, J., Tang, Y., Zheng, Z., Li, J. F., ... & Li, C. (2022). GPI-Anchored Ligand-BioID2-Tagging System Identifies Galectin-1 Mediating Zika Virus Entry. iScience, 105481.
8. Li, B., Ding, Z., Calbay, O., Li, Y., Li, T., Jin, L., & Huang, S. (2022). FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid rafts. Cancer Gene Therapy, 1-14.
9. Oh, S., Jo, S., Bajzikova, M., Kim, H. S., Dao, T. T., Rohlena, J., ... & Park, S. (2023). Non-bioenergetic roles of mitochondrial GPD2 promote tumor progression. Theranostics, 13(2), 438.
10. Zhao, Q., Su, H., Jiang, W., Luo, H., Pan, L., Liu, Y., ... & Tan, B. (2023). IGF-1 Combined with OPN Promotes Neuronal Axon Growth in Vitro Through the IGF-1R/Akt/mTOR Signaling Pathway in Lipid Rafts. Neurochemical Research, 1-12.
11. Mirza, I., Haloul, M., Hassan, C., Masrur, M., Mostafa, A., Bianco, F. M., ... & Mahmoud, A. M. (2023). Adiposomes from Obese-Diabetic Individuals Promote Endothelial Dysfunction and Loss of Surface Caveolae. Cells, 12(20), 2453.
12. Khalifa, B. A., Guijarro, A., Ravera, S., Bertola, N., Adorni, M. P., Papotti, B., ... & Nencioni, A. (2023). Cyclic fasting bolsters cholesterol biosynthesis inhibitors’ anticancer activity. Nature Communications, 14(1), 6951.
13. Cheng, M., Zhang, R., Li, J., Ma, W., Li, L., Jiang, N., ... & Wu, Z. (2024). MβCD inhibits SFTSV entry by disrupting lipid raft structure of the host cells. Antiviral Research, 106004.