Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps) – Invent Biotechnologies Inc.

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Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps)

Cat #: LR-042

  • $498.00



Manual & Protocol | Material Safety Data Sheets (MSDS)

Lipid rafts are small membrane domains containing a high level of cholesterol and sphingolipids. Lipid rafts have been found in the plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. Lipid rafts are implicated in numerous cellular processes such as signal transduction, membrane trafficking, and protein sorting.  Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+/K+ ATPase on PM. Traditional methods for lipid raft isolation involve isolation of detergent-resistant membrane subdomain from total membranous structures, which does not distinguish plasma membrane-derived and/or organelle-derived lipid rafts. Using the patented spin-column-based technologies, we have developed this kit specifically for the isolation of plasma membrane-derived lipid rafts. Larger plasma membrane vesicles are first isolated and treated with a non-ionic detergent containing buffer followed by isolation of detergent-resistant fraction by flotation centrifugation using a tabletop microcentrifuge. Highly enriched plasma membrane-derived lipid rafts can be obtained in about 1 hour without using a traditional homogenizer and ultracentrifugation.

*For total lipid raft isolation, please refer to MinuteTM Total Lipid Raft Isolation Kit under Cat # LR-039.

 

Kit Components:

Items

Quantity

Buffer A

15 ml

Buffer B

10 ml

Buffer C

10 ml

Plastic Rods

2 units

Filter Cartridge with Collection Tubes

20 units

  1. Bu, Y., Teng, Q., Feng, D., Sun, L., Xue, J., & Zhang, G. (2021). YLMY Tyrosine Residue within the Cytoplasmic Tail of Newcastle Disease Virus Fusion Protein Regulates Its Surface Expression to Modulate Viral Budding and Pathogenicity. Microbiology Spectrum9(3), e02173-21.
  2. Rashkovan, M., Albero, R., Gianni, F., Perez-Duran, P., Miller, H. I., Mackey, A. L., ... & Ferrando, A. A. (2021). Intracellular cholesterol pools regulate oncogenic signaling and epigenetic circuitries in Early T-cell Precursor Acute Lymphoblastic Leukemia. Cancer discovery.
  3. Jiang, C., Lin, Y., Shan, H., Xia, W., Pan, C., Wang, N., ... & Yu, X. (2022). miR-146a Protects against Staphylococcus aureus-Induced Osteomyelitis by Regulating Inflammation and Osteogenesis. ACS Infectious Diseases.
  4. Fiore, D., Proto, M. C., Franceschelli, S., Pascale, M., Bifulco, M., & Gazzerro, P. (2022). In Vitro Evidence of Statins’ Protective Role against COVID-19 Hallmarks. Biomedicines, 10(9), 2123.


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