Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. The majority of current commercial kits are designed to treat cultured cells and tissues similarly, not paying sufficient attention to the structural difference between the two. As a result, cross-contamination of cytosolic and nuclear fractions from fresh/frozen tissues remains a major problem (see references 1 and 2 below). This novel Cytosolic and Nuclear Extraction Kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross-contamination for fresh and frozen tissue samples.

Kit includes:

1. Li, Y., Wu, J., Yu, S., Zhu, J., Zhou, Y., Wang, P., ... & Zhao, Y. (2020). Sestrin2 promotes angiogenesis to alleviate brain injury by activating Nrf2 through regulating the interaction between p62 and Keap1 following photothrombotic stroke in rats. Brain Research, 146948.

2. Wang, B., Huang, X., Pan, X., Zhang, T., Hou, C., Su, W. J., ... & Wang, Y. X. (2020). Minocycline prevents the depressive-like behavior through inhibiting the release of HMGB1 from microglia and neurons. Brain, Behavior, and Immunity.

3. Song, T., Guan, X., Wang, X., Qu, S., Zhang, S., Hui, W., ... & Chen, X. (2021). Dynamic modulation of gut microbiota improves post‐myocardial infarct tissue repair in rats via butyric acid‐mediated histone deacetylase inhibition. The FASEB Journal, 35(3), e21385.

4. Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., ... & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.

5. Bao, J., Lu, Y., She, Q. Y., Dou, W., Tang, R., Xu, X., ... & Zheng, C. (2021). MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease. JCI insight.

6.  Luo LF, Qin LY, Wang JX, Guan P, Wang N, Ji ES. Astragaloside IV Attenuates the Myocardial Injury Caused by Adriamycin by Inhibiting Autophagy. Front Pharmacol. 2021 May 24;12:669782. doi: 10.3389/fphar.2021.669782. PMID: 34108879; PMCID: PMC8184095.

7. Yu, D., Zarate, N., Cuccu, F., Yue, J. S., Brown, T. G., Nanclares, T. N. M., ... & Gomez-Pastor, R. (2021). CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates inflammation and transcriptional dysregulation in Huntington’s disease 2. neurodegenerative diseases, 70, 71.

8. .  Weng, J., Wang, X., Xu, B., & Li, W. (2021). Augmenter of liver regeneration ameliorates ischemia-reperfusion injury in steatotic liver via inhibition of the TLR4/NF-κB pathway. Experimental and Therapeutic Medicine, 22(2), 1-8.

9. Wang, M., Wang, L., Zhou, Y., Feng, X., Ye, C., & Wang, C. (2021). Icariin attenuates renal fibrosis in chronic kidney disease by inhibiting interleukin‐1β/transforming growth factor‐β‐mediated activation of renal fibroblasts. Phytotherapy Research.

10. Yang, C., Luo, P., Chen, S. J., Deng, Z. C., Fu, X. L., Xu, D. N., ... & Liu, W. J. (2021). Resveratrol sustains intestinal barrier integrity, improves antioxidant capacity, and alleviates inflammation in the jejunum of ducks exposed to acute heat stress. Poultry Science, 101459.

11. Imai, S., Ohama, M., Suzuki, M., Katayanagi, Y., Kayashima, Y., & Tezuka, H. Research Article Short Alkyl Chain-Length Resorcinol Olivetol Protects Against Obesity with Mitochondrial Activation by Pgc-1α Deacetylation. Available at SSRN 3924601.
12. Wang, D., Wang, T., Li, Z., Guo, Y., & Granato, D. (2022). Green tea polyphenols upregulate the Nrf2 signaling pathway and suppress oxidative stress and inflammation markers in D-galactose-induced liver aging in mice. Frontiers in Nutrition, 130.

13. Lv, L., Shu, H., Mo, X., Tian, Y., Guo, H., & Sun, H. Y. (2022). Activation of the Nrf2 Antioxidant Pathway by Longjing Green Tea Polyphenols in Mice Livers. Natural Product Communications, 17(12), 1934578X221139409.

14. Wahl, D., Smith, M. E., McEntee, C. M., Cavalier, A. N., Osburn, S. C., Burke, S. D., ... & LaRocca, T. J. (2023). The reverse transcriptase inhibitor 3TC protects against age‐related cognitive dysfunction. Aging Cell, e13798.
15. Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.
16. Schmidt, A. V., Monga, S. P., Prochownik, E. V., & Goetzman, E. S. (2023). A Novel Transgenic Mouse Model Implicates Sirt2 as a Promoter of Hepatocellular Carcinoma. International Journal of Molecular Sciences, 24(16), 12618.

17. AlTamimi, J. Z., AlFaris, N. A., Alshammari, G. M., Alagal, R. I., Aljabryn, D. H., & Yahya, M. A. (2023). Esculeoside A (ESA) alleviates reproductive toxicity in streptozotocin-diabetic rats’s model by activating Nrf2 signaling. Saudi Journal of Biological Sciences, 103780.

18. ALTamimi, J. Z., AlFaris, N. A., Alshammari, G. M., Alagal, R. I., Aljabryn, D. H., & Yahya, M. A. (2023). Esculeoside A Decreases Diabetic Cardiomyopathy in Streptozotocin-Treated Rats by Attenuating Oxidative Stress, Inflammation, Fibrosis, and Apoptosis: Impressive Role of Nrf2. Medicina, 59(10), 1830.

19. Al Jadani, J. M., Albadr, N. A., Alshammari, G. M., Almasri, S. A., Alfayez, F. F., & Yahya, M. A. (2023). Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory Effects. Nutrients, 15(22), 4755.

20. .  Ryan, F., Francos-Quijorna, I., Hernández-Mir, G., Aquino, C., Schlapbach, R., Bradbury, E. J., & David, S. (2023). Tlr4 deletion modulates cytokine and extracellular matrix expression in chronic spinal cord injury, leading to improved secondary damage and functional recovery. Journal of Neuroscience.

21. Alsabaani, N. A., Amawi, K., Eleawa, S. M., Ibrahim, W. N., Aldhaban, W., Alaraj, A. M., ... & Alkhateeb, M. A. (2024). Nrf-2-dependent antioxidant and anti-inflammatory effects underlie the protective effect of esculeoside A against retinal damage in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy, 173, 116461.

22. Di Veroli, B., Bentanachs, R., Roglans, N., Alegret, M., Giona, L., Profumo, E., ... & Buttari, B. (2024). Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose. Cells, 13(15), 1247.

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