Minute™ Yeast Mitochondria Enrichment Kit (50 Preps)

Manual & Protocol | MSDS

The traditional protocol for yeast mitochondria isolation/extraction involves a range of centrifugation-based sub-cellular fractionation procedures. Typically, the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time-consuming. We feature a simple and efficient protocol for enriching yeast mitochondria. The procedure is gentle and instrument-free. Native mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation. This kit contains optimized detergent-free protein extraction buffers. The protein yield ranges from 150 to 250 μg/sample. The materials provided are sufficient for 50 extractions.

Enrichment of Mitochondria detected by Western Blotting.  Ponceau red stained blot (A) and Western blotting (B). Lane 1, Total yeast cell lysate; Lane 2, Cytosolic fraction; Lane 3 and 4, Mitochondrial fraction from two separated Experiments. Por1 is a mitochondrial marker antibody (Thermofisher, 16G9E6BC4).

Kit includes: