Catalog Number: SD-008/SN-009
Manual & Protocol | Material Safety Data Sheets (MSDS)
Minute™ Total Protein Extraction Kit for Plant Tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract denatured or native proteins from plant tissues (leaves, seeds, soft stems, roots, etc.). This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application. Because protein profiles extracted by these two methods are not identical, one might be superior to the other for various applications. With the use of the protein extraction filter cartridges, total plant-soluble proteins can be extracted from 20-200 mg plant tissue in less than 8 minutes with high protein yield (2-8 mg/ml). Extracted proteins can be used as a good starting material for protein analysis and small-scale protein purification in column chromatography.
Kit includes:
Quantity
Denaturing Lysis Buffer
25 ml
Native Lysis Buffer
Potein Extraction Filter Cartridges
50 units
Collection Tubes with Caps
Plastic Rods
2 units
Chen, J., Huang, H., Wei, S., Cui, H., Li, X., & Zhang, C. (2020). Glyphosate resistance in Eleusine indica: EPSPS overexpression and P106A mutation evolved in the same individuals. Pesticide Biochemistry and Physiology.
Tal, L., Guercio, A. M., Varshney, K., Young, A., Gutjahr, C., & Shabek, N. (2023). C-terminal conformational changes in SCF-D3/MAX2 ubiquitin ligase are required for KAI2-mediated signaling. bioRxiv, 2023-01.
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