Protein Extraction Kit for Fixed and Embedded Tissues – Invent Biotechnologies Inc.

Have a question? Ask our scientists!

{formbuilder:58797}

Minute™ Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps)

Catalog Number: FE-025

  • $315.00



Manual & Protocol | Material Safety Data Sheets (MSDS)

Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potentially rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin-mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at a high temperature with or without an optional sonication step. These methods can be effective but are tedious and time-consuming (3-4 hours). Minute™ Protein Extraction Kit for Fixed and Embedded Tissues provides a simple and rapid way to extract protein from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less than one hour with a protein yield of 2-3 mg/ml.

 

Kit includes:

Items

Quantity

Buffer A

20 ml

Buffer B

20 ml

1.5 ml Microfuge Tubes

20 units

Pestles

2 units

 

1. Gnanaguru, G., Wagschal, A., Oh, J., Saez-Torres, K. L., Li, T., Temel, R. E., ... & D’Amore, P. A. (2021). Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration. Molecular Therapy.



We Also Recommend