Shipping calculated at checkout
Couldn't load pickup availability
Questions about this product? Ask our scientist!
Minute™ Cytoplasmic and Nuclear Extraction Kits for Cells (50 Preps)
SKU:Cat #: SC-003
Manual & Protocol | MSDS
Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells comprises optimized cytoplasmic extraction buffer, nuclear protein extraction buffer, and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plants, bacteria, yeast, and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.
Kit includes:
Items |
Quantity |
Cytoplasmic Extraction Buffer |
25 ml |
Nuclear Extraction Buffer |
25 ml |
Protein Extraction Filter Cartridges |
50 units |
Collection Tubes with Caps |
50 units |
References (262)
- Kalabus, JL. et al. (2012). MicroRNAs differentially regulate carbonyl reductase 1 (CBR1) gene expression dependent on the allele status of common polymorphic variant rs9024. PLOS ONE. 7(11):1-9.
- Lombrafia AQ. et al (2014). Interindividual variability in the cardiac expression of anthracycline reductases in donors with and without down syndrome. Pharm Res. 31:1644-1655.
- Hou, N., et al. (2014). Vitamin A deficiency impairs spatial learning and memory: The mechanism of abnormal CBP-dependent histone acetylation regulated by retinoic acid receptor alpha. Mol Neurobiol. DOI 10.1007/s12035-014-8741-6.
- You, Y., et al. (2014). Latexin sensitizes leukemogenic cells to gamma-irradiation-induced cell-cycle arrest and cell death through Rps3. Cell Death and Disease. (2014)5, e1493; doi: 10.1038/cddis. 2014.443.
- Tanaka M et al. (2016). Ex vivo effects of naphthoquinones allergen-sensitized mononuclear cells in mice. International Journal of Immunopathology and Pharmacology. DOI: 10.1177/0394632016632221
- Liu W. et al. (2016) Electroacupunctre improves motor impairment via inhibition of microglia-mediated neuroinflammation in the sensorimotor cortex after ischemic stroke. Life Sciences. doi:10.1016/j.lfs.2016.01.045
- Xu, Yang, et al. (2016), RIP3 induces ischemic neuronal DNA degradation and programmed necrosis in rat via AIF." Scientific Reports 6:29362.
- Shi, Le, et al. (2016). "Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis." Tumor Biology: 1-13.9
- Huang, C. W., Feng, W., Peh, M. T., Peh, K., Dymock, B. W., & Moore, P. K. (2016). A novel slow-releasing hydrogen sulfide donor, FW1256, exerts anti-inflammatory effects in mouse macrophages and in vivo. Pharmacological Research.
- Matsuoka, Y., Nakayama, H., Yoshida, R., Hirosue, A., Nagata, M., Tanaka, T., ... & Shinriki, S. (2016). IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma. British Journal of Cancer.
- Meng, C., Cui, X., Qi, S., Zhang, J., Kang, J., & Zhou, H. (2016). Lung inflation with hydrogen sulfide during the warm ischemia phase ameliorates injury in rat donor lungs via metabolic inhibition after cardiac death. Surgery.
- Liu, Y., Peng, J., Sun, T., Li, N., Zhang, L., Ren, J., ... & Ding, Y. (2017). Epithelial EZH2 serves as an epigenetic determinant in experimental colitis by inhibiting TNFα-mediated inflammation and apoptosis. Proceedings of the National Academy of Sciences, 201700909.
- Chen, Y., Hu, X., Guo, C., Yu, Y., & Lu, H. (2017). Epe1 contributes to activation of AMPK by promoting phosphorylation of AMPK alpha subunit, Ssp2. Scientific Reports, 7.
- Hu, H., Zhu, W., Qin, J., Chen, M., Gong, L., Li, L., ... & Ye, D. (2016). Acetylation of PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology
- Hou, Y., Wang, Y., He, Q., Li, L., Xie, H., Zhao, Y., & Zhao, J. (2017). Nrf2 Inhibits NLRP3 Inflammasome Activation Through Regulating Trx1/TXNIP Complex in Cerebral Ischemia Reperfusion Injury. Behavioural Brain Research.