Nuclei and cytosol isolation kit for adipose tissues – Invent Biotechnologies Inc.
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Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Preps)

Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Preps)

Catalog Number: AN-029

  • $358.00


Manual & Protocol | Material Safety Data Sheets (MSDS)

Adipocytes are the major energy storage sites in the body and they have critical endocrine functions. Understanding the development and function of adipocytes is essential to comprehending metabolic homeostasis under physiological and pathological conditions. When it comes to adipocytes, separation of these two fractions are much more difficult because of the high concentration of lipid droplets and low protein content of adipocytes. Methods reported in the literature are tedious and time-consuming, and as much as 50 grams of tissue are required. This kit provides a very fast and simple method for obtaining high-purity nuclei from adipose tissues and, most important of all, only milligram amounts of tissues are required, making it possible to isolate nuclei and cytosol from small animal and biopsy samples. The intact nuclei isolated have many applications, such as single-nucleus RNA sequencing, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications.

Kit includes:

Items

Quantity

N/C Buffer

15 ml

1.5 ml Eppendorf Tubes

20 units

Pestle for 1.5 ml Eppendorf Tubes

2 units

Filter Cartridge

20 units

2 ml Collection Tubes

20 units


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