Minute™ Plant Golgi Apparatus Enrichment Kit (20 preps)

 Manual & Protocol | MSDS

The plant Golgi apparatus plays a critical role in the biosynthesis of cellular structural components and intracellular protein trafficking. However, proteomic characterization of the Golgi has been limited by the lack of efficient methods for obtaining enriched Golgi fractions. Traditional Golgi isolation methods rely heavily on harsh homogenization and density-gradient centrifugation, which are labor-intensive, time-consuming, and typically require large amounts of starting material.

This kit is designed to overcome these limitations by combining gentle spin column-based homogenization, differential centrifugation, and selective precipitation to enrich Golgi apparatus from plant tissues. The protocol is simple, rapid, and straightforward, requiring only milligram quantities of starting material.

Using this approach, users can achieve a 2–3-fold enrichment of the Golgi fraction while better preserving the structural integrity of the isolated Golgi apparatus than traditional isolation methods. This makes the kit well suited for downstream biochemical, proteomic, and functional analyses.

Enrichment of Golgi Apparatus from Plant Leaves

A. Ponceau S stained blot. B. Western blot with ER (BIP2), Golgi (Arf1) and histone 3 (H3) marker antibodies. The antibodies were purchased from Agrisera (Vannas, Sweden). Lanes 1&2: total protein from A. thaliana and N. tabacum; Lanes 3&4: enriched Golgi fractions from A. thaliana and N. tabacum, respectively.

Kit includes: