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Minute™ Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 preps)
SKU:Cat #: MM-038
Manual & Protocol | MSDS
Muscle cells have a large number of mitochondria because they are continually used to move the body. Another mitochondrion-rich organ is the heart, where mitochondria make up about 40% of the heart cells. However, isolating mitochondria from muscle tissue can be difficult because interfibrillar mitochondria are hidden deep within muscle fibrils. The traditional methods usually require a polytron tissue disintegrator and an ultracentrifuge for purification with a high-density medium such as a Percoll gradient. We developed this kit using our proprietary technologies, making mitochondrial isolation from muscle simple, rapid, and user-friendly. Intact mitochondria can be isolated from fresh/frozen muscle tissues in about 1 hour with higher yield.

Kit includes:
|
Items |
Quantity |
|
Buffer A |
25 mL |
|
Buffer B |
10 mL |
| Filter Cartridges |
50 units |
|
Collection tubes with cap (2 ml) |
50 units |
|
Plastic rods |
2 units |
|
Tissue dissociation beads |
5 grams |