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Minute™ Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 preps)

SKU:Cat #: MM-038

Manual & Protocol | MSDS

Muscle cells have a large number of mitochondria because they are continually used to move the body.  Another mitochondrion-rich organ is the heart, where mitochondria make up about 40% of the heart cells. However, isolating mitochondria from muscle tissue can be difficult because interfibrillar mitochondria are hidden deep within muscle fibrils. The traditional methods usually require a polytron tissue disintegrator and an ultracentrifuge for purification with a high-density medium such as a Percoll gradient. We developed this kit using our proprietary technologies, making mitochondrial isolation from muscle simple, rapid, and user-friendly. Intact mitochondria can be isolated from fresh/frozen muscle tissues in about 1 hour with higher yield.

Kit includes:

Items

Quantity

Buffer A

25 mL

Buffer B

10 mL

Filter Cartridges

50 units

Collection tubes with cap (2 ml)

50 units

Plastic rods

2 units

Tissue dissociation beads

5 grams