Minute™ Golgi Apparatus Enrichment Kit (20 Preps)

Manual & Protocol | MSDS

Golgi apparatus, also called Golgi complex or Golgi body, is made up of a series of flattened stacked pouches (cisternae). Golgi apparatus is a vital organelle in eukaryotic cells and is responsible for transporting, modifying, and packaging protein and lipids into vesicles for delivery to targeted locations.  The quantity and distribution of Golgi in different cell and tissue types vary significantly. The ability to obtain highly-enriched Golgi fraction is an important first step for studying its function and interaction with other organelles. Traditional methods for isolating Golgi apparatus are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming. Unlike any other Golgi isolation kit, this kit uses a patented spin-column-based technology that is simple, rapid and requires only a small amount of starting material. This kit can preferentially enrich native Golgi by precipitation without using a Dounce homogenizer and ultracentrifugation. Two sub-Golgi fractions can be obtained: Golgi apparatus and secretory vesicles of Golgi.

Compare with Other Golgi Apparatus Enrichment Kits

Kit Components:

1. Tan, X., Shi, L., Banerjee, P., Liu, X., Guo, H. F., Yu, J., ... & Creighton, C. J. (2020). A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. The Journal of Clinical Investigation.

2. Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.

3. Lita, A., Pliss, A., Kuzmin, A., Yamasaki, T., Zhang, L., Dowdy, T., ... & Larion, M. (2021). IDH1 mutations induce organelle defects via dysregulated phospholipids. Nature Communications, 12(1), 1-16.

4. Tan, X., Banerjee, P., Shi, L., Xiao, G. Y., Rodriguez, B. L., Grzeskowiak, C. L., ... & Kurie, J. M. (2021). p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Science Advances, 7(25), eabf4885.

5.  Tan, X., Banerjee, P., Liu, X., Yu, J., Lee, S., Ahn, Y. H., ... & Kurie, J. M. (2021). Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis. JCI insight.

6.  Zhang, L., Li, R., Geng, R., Wang, L., Chen, X. X., Qiao, S., & Zhang, G. (2022). Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway. Journal of Virology, jvi-00005.

7.  Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.

8.  Mondal, T., Shivange, G., Habieb, A., & Tushir-Singh, J. (2022). A Feasible Alternative Strategy Targeting Furin Disrupts SARS-CoV-2 Infection Cycle. Microbiology Spectrum, 10(1), e02364-21.

9.  Zhu, Y., Wu, Z., Yan, W., Shao, F., Ke, B., Jiang, X., ... & Sun, Y. (2022). Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis. EMBO molecular medicine, 14(3), e14455.

10.  Zhong, W., Lin, W., Yang, Y., Chen, D., Cao, X., Xu, M., ... & Yan, D. (2022). An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis. Nature Communications, 13(1), 1-18.

11. Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.

12.  Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

13. Tan, X., Xiao, G. Y., Wang, S., Shi, L., Zhao, Y., Liu, X., ... & Kurie, J. M. (2023). EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer. The Journal of Clinical Investigation.

14.  Liu, Y. Y., Bai, J. S., Liu, C. C., Zhou, J. F., Chen, J., Cheng, Y., & Zhou, B. (2023). The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of Virology, e00364-23.

15.  Xiao, X., Shi, J., He, C., Bu, X., Sun, Y., Gao, M., ... & Zhang, J. (2023). ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nature Communications, 14(1), 2859.

16.  Ye, G., Liu, H., Liu, X., Chen, W., Li, J., Zhao, D., ... & Huang, L. (2023). African Swine Fever Virus H240R Protein Inhibits the Production of Type I Interferon through Disrupting the Oligomerization of STING. Journal of Virology, e00577-23.

17.  Nelson, T. J., & Xu, Y. (2023). Sting and p53 DNA repair pathways are compromised in Alzheimer’s disease. Scientific Reports, 13(1), 8304.