Minute™ Nuclear Envelope Protein Extraction Kit (50 Preps)

Manual & Protocol | MSDS

The nuclear envelope is a complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional nuclear envelope isolation and purification methods require large amounts of starting material and involve lengthy, tedious procedures. Invent Biotechnologies MinuteTM nuclear envelope protein extraction kit is the first commercial kit designed to rapidly isolate the nuclear envelope and its associated proteins in native form without using density-gradient centrifugation and ultracentrifugation. Due to the use of protein extraction filter cartridges, nuclear envelope protein isolation is simple, easy, and user-friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional methods, this kit starts with only 10-20 million cells and uses detergent- and EDTA-free buffers.  The procedure can be completed in under 45 minutes, with a final yield of 10-50 µg protein/sample.

A. SDS-PAGE profiles and Western blottings of isolated mouse spleen cells. Lanes 1 and 2, total cell lysate; Lane 3, nuclear envelope.

B. SDS-PAGE profiles and Western blottings of Rate (REL-6TN) and human (MCF-7) cell lines. Lane 1, REL-6TN total cell lysate; Lane 2, REL-6TN nuclear envelope. Lane 3, MCF-7 total cell lysate; Lane 4, MCF-7 nuclear envelope. Nuclear envelope marker Lamin B1 (ab 16048, ABcam, Cambridge MA) and GAPDH (G9545, Sigma) were visualized by a color metric substrate (Bio-Rad).

Kit includes: