Minute™ ER Enrichment Kit (20 Preps)

Manual & Protocol | MSDS

The endoplasmic reticulum (ER) is a major membranous structure that functionally connects the nuclear membrane and plasma membrane. ER plays a central role in the exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in the cytoplasm are translocated into ER from where vesicles shuttle protein cargo to the Golgi apparatus, which subsequently fuse with the plasma membrane. Traditional methods for isolating ER are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming with significant cross-contamination. Currently, all commercial kits for ER isolation are based on the methods developed in the 1970s of the last century. Unlike any other ER isolation kit in the market, the Minute^TM ER enrichment kit employs a patented spin-column-based technology that is simple, rapid, and requires only a small amount of starting cultured cells or tissues. This kit can differentially precipitate native ER (mainly rough ER) from cultured cells/tissues without using a Dounce homogenizer and ultracentrifugation. The whole protocol can be done in about 2h.

Compare with Other ER Isolation Kits

1. Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.
2. Dowdy, T., Zhang, L., Celiku, O., Movva, S., Lita, A., Ruiz-Rodado, V., ... & Larion, M. (2020). Sphingolipid Pathway as a Source of Vulnerability in IDH1mut Glioma. Cancers, 12(10), 2910.
3. Lita, A., Pliss, A., Kuzmin, A., Yamasaki, T., Zhang, L., Dowdy, T., ... & Larion, M. (2021). IDH1 mutations induce organelle defects via dysregulated phospholipids. Nature Communications, 12(1), 1-16.
4. Zhang, L., Li, R., Geng, R., Wang, L., Chen, X. X., Qiao, S., & Zhang, G. (2022). Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway. Journal of Virology, jvi-00005.
5. Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.
6. Mondal, T., Shivange, G., Habieb, A., & Tushir-Singh, J. (2022). A Feasible Alternative Strategy Targeting Furin Disrupts SARS-CoV-2 Infection Cycle. Microbiology Spectrum, 10(1), e02364-21.
7. Zhu, Y., Wu, Z., Yan, W., Shao, F., Ke, B., Jiang, X., ... & Sun, Y. (2022). Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis. EMBO molecular medicine, 14(3), e14455.
8. Wang, Y., Cao, X., Liu, P., Zeng, W., Peng, R., Shi, Q., ... & Wang, H. (2022). KCTD7 mutations impair the trafficking of lysosomal enzymes through CLN5 accumulation to cause neuronal ceroid lipofuscinoses. Science Advances, 8(31), eabm5578.

9.  Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.

10. Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.
11. Chiu, C. C., Weng, Y. H., Yeh, T. H., Lu, J. C., Chen, W. S., Li, A. H. R., ... & Wang, H. L. (2023). Deficiency of RAB39B Activates ER Stress-Induced Pro-apoptotic Pathway and Causes Mitochondrial Dysfunction and Oxidative Stress in Dopaminergic Neurons by Impairing Autophagy and Upregulating α-Synuclein. Molecular Neurobiology, 1-23.
12. Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
13.  Liu, Y. Y., Bai, J. S., Liu, C. C., Zhou, J. F., Chen, J., Cheng, Y., & Zhou, B. (2023). The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of Virology, e00364-23.
14. Xiao, X., Shi, J., He, C., Bu, X., Sun, Y., Gao, M., ... & Zhang, J. (2023). ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nature Communications, 14(1), 2859.
15. Li, Y., Li, K., Pan, T., Xie, Q., Cheng, Y., Wu, X., ... & Cui, S. (2023). Translocation of IGF-1R in endoplasmic reticulum enhances SERCA2 activity to trigger Ca2+ ER perturbation in hepatocellular carcinoma. Acta Pharmaceutica Sinica B.
16. Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.