Minute™ Single Nucleus Isolation Kit for Tissues/Cells

Manual & Protocol | Material Safety Data Sheets (MSDS)

*Invent Biotechnologies, Inc. is the inventor of the spin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the technology. 

Isolation and purification of nuclei from tissues and cultured cells are common lab practices. Isolated nuclei, especially single nucleus, are widely used for a variety of experiments such as FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and apoptosis research. The traditional method for the isolation of nuclei is relatively simple. However, nucleus aggregation and formation of a high percentage of doublets are frequently encountered problems. This kit is specially designed to address these issues. The protocol is simple, rapid, and very effective. A high percentage of the well-separated single nucleus can be obtained in about 30 min. In comparison to the traditional method, the kit requires less starting material and has a larger range of sample sizes (1-30 mg). The buffers contain a proprietary mix of detergents for efficient cell lysis. If the presence of detergent is not desired, a detergent-free nuclei isolation kit is also available under cat# NI-024

For storage and transportation of isolated single nuclei, the following buffer is recommended:

WA-014: Minute™ Anti-Clumping Nuclei Storage Buffer 

NOTE: We also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:

NI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit

BN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

AN-029: Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes

Kit includes:

1. Kang, R. B., Li, Y., Rosselot, C., Zhang, T., Siddiq, M., Rajbhandari, P., ... & Lu, G. (2022). Single Nucleus RNA Sequencing of Human Pancreatic Islets In Vitro and In Vivo Identifies New Gene Sets and Three β-Cell Subpopulations with Different Transcriptional Profile. bioRxiv.
2. de Nicola, F., Corleone, G., & Goeman, F. (2022). Dissecting the Epigenome Driving Drug Resistance by. In Cancer Drug Resistance (pp. 171-185). Humana, New York, NY.
3. Kang, R. B., Li, Y., Rosselot, C., Zhang, T., Siddiq, M., Rajbhandari, P., ... & Lu, G. (2023). Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles. Genome Medicine, 15(1), 1-24.
4. Takeuchi, F., Liang, Y. Q., Shimizu-Furusawa, H., Isono, M., Ang, M. Y., Mori, K., ... & Kato, N. (2023). Gene-regulation modules in nonalcoholic fatty liver disease revealed by single-nucleus ATAC-seq. Life Science Alliance, 6(10).
5. Tang, X., Lynn, G. E., Cui, Y., Cerny, J., Arora, G., Tomayko, M. M., ... & Fikrig, E. (2023). Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite. Infection and Immunity, e00282-23.
6. Kang, R. B., Lee, J., Varela, M., Li, Y., Rosselot, C., Zhang, T., ... & Lu, G. (2023). Human Pancreatic α-Cell Heterogeneity and Trajectory Inference Analysis Using Integrated Single Cell-and Single Nucleus-RNA Sequencing Platforms. bioRxiv, 2023-11. 

    

7. Tyshkovskiy, A., Kholdina, D., Ying, K., Davitadze, M., Molière, A., Tongu, Y., ... & Gladyshev, V. N. (2024). Transcriptomic Hallmarks of Mortality Reveal Universal and Specific Mechanisms of Aging, Chronic Disease, and Rejuvenation. bioRxiv, 2024-07.