Minute™ Single Nucleus Isolation Kit for Tissues/Cells

Manual & Protocol | Material Safety Data Sheets (MSDS)

Isolation and purification of nuclei from tissues and cultured cells are common lab practices. Isolated nuclei, especially single nucleus, are widely used for a variety of experiments such as FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and apoptosis research. The traditional method for the isolation of nuclei is relatively simple and straightforward. However, nucleus aggregation and formation of a high percentage of doublets are frequently encountered problems. This kit is specially designed to address these issues. The protocol is simple, rapid, and very effective. A high percentage of the well-separated single nucleus can be obtained in about 30 min. In comparison to the traditional method, the kit requires less starting material and has a larger range of sample size (1-30 mg). The buffers contain a proprietary mix of detergents for efficient cell lysis. If the presence of detergent is not desired, a detergent-free nuclei isolation kit is also available under cat# NI-024

For storage and transportation of isolated single nuclei, the following buffer is recommended:

WA-014: Minute™ Anti-Clumping Nuclei Storage Buffer 

NOTE: We also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:

NI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit

BN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

AN-029: Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes

Kit includes: