Minute™ ER Enrichment Kit (20 Preps)

Manual & Protocol | MSDS

The endoplasmic reticulum (ER) is a major membranous structure that functionally connects the nuclear membrane and plasma membrane. ER plays a central role in the exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in the cytoplasm are translocated into ER from where vesicles shuttle protein cargo to the Golgi apparatus, which subsequently fuse with the plasma membrane. Traditional methods for isolating ER are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming with significant cross-contamination. Currently, all commercial kits for ER isolation are based on the methods developed in the 1970s of the last century. Unlike any other ER isolation kit in the market, the Minute^TM ER enrichment kit employs a patented spin-column-based technology that is simple, rapid, and requires only a small amount of starting cultured cells or tissues. This kit can differentially precipitate native ER (mainly rough ER) from cultured cells/tissues without using a Dounce homogenizer and ultracentrifugation. The whole protocol can be done in about 2h.

Compare with Other ER Isolation Kits

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