Minute™ ER Enrichment Kit (20 Preps)

Manual & Protocol | MSDS

The endoplasmic reticulum (ER) is a significant membranous structure that bridges the nuclear membrane and the plasma membrane, serving as a pivotal player in the exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in the cytoplasm are directed to the ER, from where vesicles transport protein cargo to the Golgi apparatus, leading to subsequent fusion with the plasma membrane.

Traditional ER isolation methods rely on density gradient ultracentrifugation, a process demanding a substantial amount of starting material. These methods can be laborious, time-consuming, and often result in notable cross-contamination. Remarkably, all existing commercial kits for ER isolation are based on techniques developed in the 1970s. In a departure from these traditional approaches, the Minute™ ER enrichment kit stands out in the market by harnessing patented spin-column-based technology. This innovative method is not only straightforward and rapid but also requires only a small quantity of starting cultured cells or tissues.

This kit excels at differentially precipitating native ER, primarily rough ER, from cultured cells and tissues, all without the need for a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in approximately two hours.

Compare with Other ER Isolation Kits

1. Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.
2. Dowdy, T., Zhang, L., Celiku, O., Movva, S., Lita, A., Ruiz-Rodado, V., ... & Larion, M. (2020). Sphingolipid Pathway as a Source of Vulnerability in IDH1mut Glioma. Cancers, 12(10), 2910.
3. Lita, A., Pliss, A., Kuzmin, A., Yamasaki, T., Zhang, L., Dowdy, T., ... & Larion, M. (2021). IDH1 mutations induce organelle defects via dysregulated phospholipids. Nature Communications, 12(1), 1-16.
4. Zhang, L., Li, R., Geng, R., Wang, L., Chen, X. X., Qiao, S., & Zhang, G. (2022). Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway. Journal of Virology, jvi-00005.
5. Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.
6. Mondal, T., Shivange, G., Habieb, A., & Tushir-Singh, J. (2022). A Feasible Alternative Strategy Targeting Furin Disrupts SARS-CoV-2 Infection Cycle. Microbiology Spectrum, 10(1), e02364-21.
7. Zhu, Y., Wu, Z., Yan, W., Shao, F., Ke, B., Jiang, X., ... & Sun, Y. (2022). Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis. EMBO molecular medicine, 14(3), e14455.
8. Wang, Y., Cao, X., Liu, P., Zeng, W., Peng, R., Shi, Q., ... & Wang, H. (2022). KCTD7 mutations impair the trafficking of lysosomal enzymes through CLN5 accumulation to cause neuronal ceroid lipofuscinoses. Science Advances, 8(31), eabm5578.

9.  Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.

10. Ruiz-Rodado, V., Lita, A., & Larion, M. (2022). Advances in measuring cancer cell metabolism with subcellular resolution. Nature Methods, 1-16.
11. Chiu, C. C., Weng, Y. H., Yeh, T. H., Lu, J. C., Chen, W. S., Li, A. H. R., ... & Wang, H. L. (2023). Deficiency of RAB39B Activates ER Stress-Induced Pro-apoptotic Pathway and Causes Mitochondrial Dysfunction and Oxidative Stress in Dopaminergic Neurons by Impairing Autophagy and Upregulating α-Synuclein. Molecular Neurobiology, 1-23.
12. Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.
13.  Liu, Y. Y., Bai, J. S., Liu, C. C., Zhou, J. F., Chen, J., Cheng, Y., & Zhou, B. (2023). The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of Virology, e00364-23.
14. Xiao, X., Shi, J., He, C., Bu, X., Sun, Y., Gao, M., ... & Zhang, J. (2023). ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nature Communications, 14(1), 2859.
15. Li, Y., Li, K., Pan, T., Xie, Q., Cheng, Y., Wu, X., ... & Cui, S. (2023). Translocation of IGF-1R in endoplasmic reticulum enhances SERCA2 activity to trigger Ca2+ ER perturbation in hepatocellular carcinoma. Acta Pharmaceutica Sinica B.
16. Zhu, Y., Lei, L., Wang, X., Jiang, Q., Loor, J. J., Kong, F., ... & Li, X. (2023). Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver. Journal of Dairy Science.
17. Zhou, Q., Yang, Y., Xu, Z., Deng, K., Zhang, Z., Hao, J., ... & Li, Y. P. (2023). ATAD1 inhibits hepatitis C virus infection by removing the viral TA‐protein NS5B from mitochondria. EMBO reports, e56614.
18. Wang, W., Tan, J., Liu, X., Guo, W., Li, M., Liu, X., ... & Zhou, Q. (2023). Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress. Nature Communications, 14(1), 6201.