Minute™ Chloroplast Isolation Kit (50 Preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Invent Biotechnologies Minute^TM chloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.). Due to the use of filter cartridges with pre-defined pore size and thickness, intact chloroplasts can be isolated from 50-200 mg fresh plant tissues in less than 5 min. Unlike many other methods that require 1-10+ gram tissues for chloroplast isolation, this kit can quickly obtain 1 X 10⁶ to 1 X 10⁷ intact chloroplasts (>90% intact) from fresh plant leaves.

Kit includes: 

1. H Oh,. Et al. (2015) Two complete chloroplast genome sequences of Cannabis sativa varieties Mitochondrial DNA. DOI:10.3109/19401736.2015.1053117.
2. Hu, D., Li, Y., Jin, W., Gong, H., He, Q., & Li, Y. (2017). Identification and Characterization of a Plastidic Adenine Nucleotide Uniporter (OsBT1-3) Required for Chloroplast Development in the Early Leaf Stage of Rice. Scientific Reports, 7, 41355.
3. Kanazawa, A., Ostendorf, E., Kohzuma, K., Hoh, D., Strand, D. D., Sato-Cruz, M., ... & Kramer, D. M. (2017). Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force: Implications for Photosystem I and Photosystem II Photoprotection. Frontiers in Plant Science, 8, 719.
4. Suzuki, J.Y., Geib, S.M., Carlsen, M.M., Henriquez, C.L., Amore, T.D., Sim, S.B., Matsumoto, T.K., Keith, L.M. and Myers, R.Y. (2017). Development of chloroplast single nucleotide polymorphisms (SNPs) as a tool towards interspecies typing of Anthurium germplasm. Acta Hortic. 1167, 257-270
5. ShuoHao, H., Jing, L., Jie, Z., JianYun, Z., & LongQuan, H. (2018). Identification and characterization of a pyridoxal 5′-phosphate phosphatase in tobacco plants. Plant Science.
6. Ma, F., Yao, W., Wang, L., & Wang, Y. (2019). Dynamic translocation of stilbene synthase VpSTS29 from a Chinese wild Vitis species upon UV irradiation. Phytochemistry, 159, 137-147.
7. Ren, R. C., Wang, L. L., Zhang, L., Zhao, Y. J., Wu, J. W., Wei, Y. M., ... & Zhao, X. Y. (2020). DEK43 is a P‐type pentatricopeptide repeat (PPR) protein responsible for the Cis‐splicing of nad4 in maize mitochondria. Journal of Integrative Plant Biology, 62(3), 299-313.
8. Lee, S., Joung, Y. H., Kim, J. K., Do Choi, Y., & Jang, G. (2019). An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development. BMC Plant Biology, 19(1), 524.
9. Yin, B., Zhang, J., Liu, Y., Pan, X., Zhao, Z., Li, H., ... & Liu, D. (2019). PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr. Scientific Reports, 9(1), 1-15.
10. Wang, C., Chen, S., Dong, Y., Ren, R., Chen, D., & Chen, X. Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice. New Phytologist.
11. Shoeva, O. Y., Mursalimov, S. R., Gracheva, N. V., Glagoleva, A. Y., Börner, A., & Khlestkina, E. K. (2020). Melanin formation in barley grain occurs within plastids of pericarp and husk cells. Scientific Reports, 10(1), 1-9.
12. Long, J., Xing, W., Wang, Y., Wu, Z., Li, W., Zou, Y., ... & Zhang, F. (2021). Comparative Proteomic Analysis on Chloroplast Proteins Provides New Insights Into the Effects of Low Temperature in Sugar Beet.
13. Shapiro, K. O. (2021). Plug and play: Is “directed endosymbiosis” of chloroplasts possible?. bioRxiv.
14. Pan, T., Liu, Y., Ling, C., Tang, Y., Tang, W., Liu, Y., ... & Wang, S. (2021). CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC. bioRxiv.
15. Li, C., Li, J., Du, X., Zhang, J., Zou, Y., Liu, Y., ... & Lu, H. (2022). Chloroplast Thylakoidal Ascorbate Peroxidase, PtotAPX, Has Enhanced Resistance to Oxidative Stress in Populus tomentosa. International Journal of Molecular Sciences, 23(6), 3340.
16. Cao, S. K., Liu, R., Wang, M., Sun, F., Sayyed, A., Shi, H., ... & Tan, B. C. (2022). The small PPR protein SPR2 interacts with PPR-SMR1 to facilitate the splicing of introns in maize mitochondria. Plant Physiology.
17. Bélanger, S., Kramer, M. C., Payne, H. A., Hui, A. Y., Slotkin, R. K., Meyers, B. C., & Staub, J. M. (2023). Plastid dsRNA transgenes trigger phased small RNA-based gene silencing of nuclear-encoded genes. The Plant Cell, 35(9), 3398.
    
18. Liu, L., He, X., Wang, S., Qin, X., Che, S., Wu, L., ... & Yang, M. (2023). The role of the chloroplast localised phosphate transporter GmPHT4; 10 gene in plant growth, photosynthesis and drought resistance. Functional Plant Biology.
19. Wang, Y. Q., Ye, J. J., Yang, H. Z., Li, D., Li, X. X., Wang, Y. K., ... & Lu, J. L. (2023). Shading-Dependent Greening Process of the Leaves in the Light-Sensitive Albino Tea Plant ‘Huangjinya’: Possible Involvement of the Light-Harvesting Complex II Subunit of Photosystem II in the Phenotypic Characteristic. International Journal of Molecular Sciences, 24(12), 10314.
20. Nworji, O., Ogana, J., Orji, E., Ezeigwe, O., & Enemor, A. (2023). The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis.
21. Pan, T., Liu, Y., Hu, X., Li, P., Lin, C., Tang, Y., ... & Wang, S. (2023). Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC. Cell Reports, 42(10).
22. The, S. V., Santiago, J. P., Pappenberger, C., Hammes, U. Z., & Tegeder, M. (2023). UMAMIT44 is a key player in glutamate export from Arabidopsis chloroplasts. The Plant Cell, koad310.
    
23. Wang, X., Yang, J., Hu, H., Yuan, T., Zhao, Y., Liu, Y., ... & Liu, J. (2024). Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana. Viruses, 16(4), 489.
24. Sun, X., Singla-Rastogi, M., Wang, J., Zhao, C., Wang, X., & Li, P. (2024). The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids. Nucleic Acids Research, gkae339.

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