Minute™ Lipid Raft Isolation Kit for Mammalian Cells/Tissues – Invent Biotechnologies Inc.
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Minute™ Lipid Raft Isolation Kit for Mammalian Cells/Tissues

Catalog Number: LR-039

  • $398.00


Manual & Protocol Material Safety Data Sheets (MSDS)

Lipid rafts are small membrane domains containing high level of cholesterol and sphingolipids.  Lipid rafts are found in plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. They have been implicated in numerous cellular processes including but not limited to signal transduction, membrane trafficking and protein sorting. Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+/K+ ATPase on plasma membrane. Lipid rafts are traditionally isolated by sucrose-gradient or OptiPrep gradient using ultracentrifugation that requires large amount of starting material. The protocol is tedious and time consuming. This kit was developed using our proprietary spin-column-based technologies, offering a rapid and easy way to isolate lipid rafts. Total membrane fraction (include PM and organelle membranes) is first isolated and treated with a non-ionic detergent containing buffer, followed by isolation of detergent resistant fraction by flotation centrifugation using just a table top microcentrifuge. Highly enriched total lipid rafts can be obtained from cultured cells/tissues in less than 90 min without using density gradient and ultracentrifugation.

Kit includes:

Items

Quantity

Buffer A

15 ml

Buffer B

10 ml

Buffer C 10 ml

Filter Cartridges

20 units

Collection Tubes (2 ml)

20 units

 


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