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Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)

Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)

Catalog Number: CS-031

  • $198.00


Manual & Protocol | Material Safety Data Sheets (MSDS)

Obtaining quality cell suspension from fresh and/or frozen tissues is an important first step for flow cytometry analysis (FACS) and nucleic acid purification. Cell suspensions can be isolated from tissues by one or a combination of the following three mechanisms: chemical tissue dissociation, enzymatic digestion and physical separation. Many methods are tedious and time-consuming, but most importantly, cells in the tissue, especially frozen tissue, are usually damaged and show low integrity and viability. It is difficult to obtain high-viability cell suspension from frozen tissues with high connective tissue contents such as liver, kidney and brain tissues. We have developed a simple and fast method for isolating cell suspension from fresh or frozen tissues using a combination of chemical tissue dissociation and physical separation mechanisms. The protocol can be finished in about 20 minutes with high cell integrity and viability.

Kit includes:

Items

Quantity

Tissue Dissociation Buffer

15 ml

1.5 ml Microfuge Tube

50 units

Pestles for 1.5 ml Tube

2 units


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