Endosome isolation and cell fractionation spin column based – Invent Biotechnologies Inc.
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Minute™ Endosome Isolation and Cell Fractionation Kit (20 Preps)

Catalog Number: ED-028

  • $468.00


Manual & Protocol | Material Safety Data Sheets (MSDS)

Early endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues.

Kit includes:

Items

Quantity

Buffer A

15 ml

Buffer B

15 ml

Plastic Rods

2 units

Filter Cartridge

20 units

Tissue Dissociation Beads

2.5 grams

Collection Tubes

20 units

 

  1. Xu et al., (2018) Phosphorylation-Mediated IFN-gR2 MembraneTranslocation Is Required to Activate Macrophage Innate Response.  Cell 175, 1–16.
  2. Drelich, A., Judy, B., He, X., Chang, Q., Yu, S., Li, X., ... & Ksiazek, T. (2018). Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells. Viruses10(10), 563.

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