Minute™ Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Prep – Invent Biotechnologies Inc.

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Minute™ Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps)

Catalog Number: SC-012

  • $298.00



Manual & ProtocolMaterial Safety Data Sheets (MSDS)

MinuteTM single cell isolation kit comprises optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to isolate single cells from fresh animal tissues rapidly, and it can also isolate nuclei from tissues briefly fixed with formaldehyde for chromosome immunoprecipitation (ChIP). The tissue disaggregation buffers are formulated to disaggregate animal tissues gently. The buffers don’t contain any proteinases and EDTA that may adversely affect cell surface markers. Due to filter cartridges with pre-defined pore size and a specially formulated buffer system, single-cell suspension can be obtained from fresh tissues in less than 10 min. This kit is specifically designed for lymphoid tissues such as the spleen, thymus, and lymph node. For other tissues, the performance is tissue type-dependent. For cell suspension isolation from frozen/fresh tissues, please refer to Cat# CS-031.

 

Kit includes:

Items

Quantity

Buffer A (for non-fixed tissues)

25 ml

Buffer B (for fixed tissues)

25 ml

Cell Isolation Filter Cartridges

50 units

Collection Tubes with Caps

50 units

Plastic Rods

2 units

 

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  7. Günther, H. S., Henne, S., Oehlmann, J., Urban, J., Pleizier, D., Renevier, N., ... & Wülfing, C. (2021). GFAP and desmin expression in lymphatic tissues leads to difficulties in distinguishing between glial and stromal cells. Scientific Reports, 11(1), 1-14.
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