Plasma Membrane, Protein Extraction and Cell Fractionation – Invent Biotechnologies Inc.

Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (50 Preps)

Catalog Number: SM-005

  • $398.00

Manual & Protocol Material Safety Data Sheets (MSDS)

The MinuteTM kit represents the next generation product for plasma membrane (PM) isolation and cell fractionation, offering unsurpassed convenience and consistency by eliminating uncontrollable variations associated with traditional homogenization and density gradient centrifugation including two phase partitioning.

How it works: cells/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied, resulting in a cell lysate containing ruptured cell membranes and intact nuclei. As a result, the nuclear contaminations are virtually eliminated. PM is further separated from the cell lysate (a mixture of crude membranes, intact nuclei, cytosol proteins and organelles) by subsequent differential and density centrifugation with a regular tabletop microcentrifuge. 5 distinct cell fractions (total membrane, PM, cytosol, nucleus and organelles) can be obtained at the completion of the protocol. The procedure can be completed in less than 45 minutes.


Figure 1. A. SDS-PAGE profiles of total cell lysate (TC) and isolated plasma membrane proteins (PM) from human and rat cultured cells. Lane 1, Human lung cancer cell A549 total cell lysate; Lane 2, Isolated PM of A549; Lane 3, PM of rat REL-6TN cells; Lane 4, Total cell lysate of rat REL-6TN cells.

B. Western blotting: Proteins in A were transferred to nitrocellulose membrane and probed with anti-Na/K ATPase alpha1, a plasma membrane marker (Upstate, Clone 464.6) and anti-lamin B1, a nuclear envelope marker (ab16048, abcam Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD).

C. Densitometry measurement of Na/K ATPase alpha1 signals in B (TC vs. PM). Total cell lysates were extracted by Minute Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Eden Prairie, MN).

Figure 2 A. SDS-PAGE profiles of isolated total membrane proteins from mouse tissues (T=Total Cell Lysate, C=Cytosol Fraction, M= Total Membrane Fraction)

B. Proteins shown in A were transferred to a nitrocellulose membrane and probed with rabbit-anti mouse pan-cadherin (ab6529, abcam, Cambridge, MA), and anti-actin by Western blotting. The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). Signals of pan-cadherin (a 130 kDa plasma membrane marker) were significantly enhanced in total membrane protein fractions. Total cell lysates were extracted by Minute™ Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Eden Prairie, MN).


Figure 3. A. SDS-PAGE profiles of total membrane protein fraction (TM) and isolated plasma membrane protein fraction from mouse tissues. 

B. Western blotting of proteins in A were transferred to nitrocellulose membrane and probed with rabbit anti-cadherin (abcam, Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). 

C. Densitometry measurement of cadherin signals in B (TM vs. PM).


Kit includes:



Buffer A

25 ml

Buffer B

10 ml

Protein Extraction Filter Cartridges

50 units

Collection Tubes with Caps

50 units

Plastic Rods

2 units

Tissue Dissociation Beads

10 grams


  1. Kalabus JL, et al. (2013). Viral Attachment Induces Rapid Recruitment of an Innate Immune Sensor (TRIM5α) to the Plasma Membrane. J Innate Immun 5:414-424.
  2. Wen D. et al. (2013). Regulation of BK- expression in the distal nephron by aldosterone and urine pH. Am J Physiol Renal Physiol. 305: F463-F476.
  3. Yang J. et al (2014). PLCy1-PKCy signaling-mediated Hsp90a plasma membrane translocation facilitates tumor metastasis. Traffic; 15(8): 861-78.
  4. Marry E.W. et al (2014). Filamin-A is required for the incorporation of tissue factor into cell-derived microvesicles. Thromb Haemost; 111:647-655.
  5. Kutluay, S., et al. (2014). Global Changes in the RNA Binding Specificity of HIV-1 Gag Regulate Virion Genesis, Cell (2014),
  6. Leung W-H. et al.(2015). PRL-3 Mediates the protein Maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.The Journal of Immunology.doi:10.4049/jimmunol.1400817.
  7. Henry E., et al. (2015). Beyond Glysolysis: GAPDHs are multifunctional enzymes involved in regulation of ROS, autophage, and plant immune responses. PLoS Genet 11(4): e1005199. doi:10.1371/journal. pgen.1005199.
  8. Wen D., et al. (2015). Increased epithelial sodium channel activity contributes to hypertension caused by Na+-Hco- cotransporter electrogenic 2 defficiency. Hypertension. 2015;66:00-00. DOI: 10.1161/HYPERTENSIONAHA.115.05394.
  9. Yamaguchi T., et al. (2015). Expression of BAF57 in ovarian cancer cells and drug sensitivity.Cancer Sci 106 (2015) 359–366. doi: 10.1111/cas.12612.
  10. Wen D. et al. (2015). Deficient acid handling with distal RTA in the NBCe2 knockout mouse. Am J Physiol Renal Physiol (June 24, 2015). doi:10.1152/ajprenal.00163.2015.
  11. Kashiwagi Y, Nagoshi T, Yoshino T, Tanaka TD, Ito K, Harada T, et al. (2015) Expression of SGLT1 in Human Hearts and Impairment of Cardiac Glucose Uptake by Phlorizin during Ischemia- Reperfusion Injury in Mice. PLoS ONE 10(6):e0130605. doi:10.1371/journal.pone.0130605.
  12. Georgiou D. K., et al. (2015). Ca2+ Binding/Permeation via Calcium Channel, CaV1.1, Regulates the Intracellular Distribution of the Fatty Acid Transport Protein, CD36, and Fatty Acid Metabolism doi: 10.1074/jbc.M115.643544 jbc.M115.643544.
  13. Devarakonda C. V. et al.(2015). Patient-derived heavy chain antibody targets cell surface HSP90 on breast tumors. BMC Cancer 2015, 15:614 doi:10.1186/s12885-015-1608-z
  14. Lin Z-J, D.,et al(2015) PBL13 is a serine/threonine protein kinase that negatively regulates Arabidopsis immune responses. Plant Physiology. doi:
  15. Mickle, A. D., Shepherd, A. J., Loo, L., & Mohapatra, D. P. (2015). Induction of Thermal and Mechanical Hypersensitivity by Parathyroid Hormone-related Peptide (PTHrP) Through Upregulation of TRPV1 Function and Trafficking. Pain, 156(9), 1620.
  16. Izumi D., et al. (2016). CXCL12/CXCR4 activation by cancer-associated
  17. fibroblasts promotes integrin b1 clustering and invasiveness in gastric cancer. Int. J. Cancer: 138, 1207–1219.
  18. Sebla B. Kutluay and Paul D. Bieniasz. (2016) Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq. Methods in Mol. Biol. 1354, pp 119-131.
  19. Mukai H., et al. (2016) PKN3 is the major regulator of angiogenesis and tumor metastasis in mice. Scientific Reports | 6:18979 | DOI: 10.1038/srep18979.
  20. Tao JQ et al. (2016). Onset of Inflammation with Ischemia: Implications for Donor Lung Preservation and Transplant Survival. American Journal of Transplantation. doi: 10.1111/ajt.13794.
  21. Vazquez-Medina J.P. et al. (2016). The phospholipase A2 activity of peroxiredoxin 6 modulates NADPH oxidase 2 activation vialysophosphatidic acid receptor signaling in the pulmonary endothelium and alveolar macrophages. The FASEB Journal. doi: 10.1096/fj.201500146R.
  22. Lin W. et al.,( 2016). Syndecan-4 negatively regulates antiviral signaling by mediating RIG-I deubiquitination via CYLD. Nature communications, DOI: 10.1038/ncomms11848.
  23. Hernández-Saavedra, Daniel, et al.(2016) "RTP801 Amplifies Nox-4-dependent Oxidative Stress Induced by Cigarette Smoke." American Journal of Respiratory Cell and Molecular Biology.
  24. LEE, S. J., PARK, B. N., ROH, J. H., AN, Y. S., HUR, H., & YOON, J. K. (2016). B Enhancing the Therapeutic Efficacy of 2-Deoxyglucose in Breast Cancer Cells Using Cell-cycle Synchronization. Anticancer Research, 36(11), 5975-5980.
  25. Choi, K. M., Kim, W., Hong, J. T., & Yoo, H. S. (2017). Dodeca-2 (E), 4 (E)-dienoic acid isobutylamide enhances glucose uptake in 3T3-L1 cells via activation of Akt signaling. Molecular and Cellular Biochemistry, 426:9-15
  26. Hisamatsu, Y., Suzuki, N., Masum, A. A., Shibuya, A., Abe, R., Sato, A. & Aoki, S. (2017). Cationic Amphiphilic Tris-Cyclometalated Iridium (III) Complexes Induce Cancer Cell Death via Interaction with Ca2+-Calmodulin Complex. Bioconjugate Chemistry. 28:507-523
  27. Sun, X., Tao, L., Yi, L., Ouyang, Y., Xu, N., Li, D., & Zhang, Z. (2017). N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database. Journal of Pharmaceutical Analysis.
  28. Luo, Y., Duan, H., Qian, Y., Feng, L., Wu, Z., Wang, F., ... & Yan, X. (2017). Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis. Cell Research, 27(3), 352-372.
  29. Zheng, J., Zhao, Y., Lun, Q., Song, Y., Shi, S., Gu, X., ... & Tu, P. (2017). Corydalis edulis Maxim. Promotes Insulin Secretion via the Activation of Protein Kinase Cs (PKCs) in Mice and Pancreatic β Cells. Scientific Reports, 7, 40454.
  30. Hoshi, Y., Uchida, Y., Tachikawa, M., Ohtsuki, S., & Terasaki, T. (2017). Actin filament‐associated protein 1 (AFAP‐1) is a key mediator in inflammatory signaling‐induced rapid attenuation of intrinsic P‐gp function in human brain capillary endothelial cells. Journal of Neurochemistry, 141(2):247-262.
  31. Xu, N., Hirohama, D., Ishizawa, K., Chang, W. X., Shimosawa, T., Fujita, T. & Shibata, S. (2017). Hypokalemia and Pendrin Induction by Aldosterone. Hypertension, HYPERTENSINAHA-116.
  32. Hayashiuchi, M., Kitayama, T., Morita, K. et al. J Anesth (2017) General anesthetic actions on GABAA receptors in vivo are reduced in phospholipase C-related catalytically inactive protein knockout mice. Journal of Anesthesia, 1-8. doi:10.1007/s00540-017-2350-2.
  33. Gong, B., Shen, W., Xiao, W., Meng, Y., Meng, A., & Jia, S. (2017). The Sec14-like phosphatidylinositol transfer proteins Sec14l3/SEC14L2 act as GTPase proteins to mediate Wnt/Ca2+ signaling. eLife, 6.
  34. Ueda, K., Nishimoto, M., Hirohama, D., Ayuzawa, N., Kawarazaki, W., Watanabe, A., ... & Fujita, T. (2017). Renal Dysfunction Induced by Kidney-Specific Gene Deletion of Hsd11b2 as a Primary Cause of Salt-Dependent Hypertension. Hypertension, HYPERTENSIONAHA-116.
  35. Asano, S., Taniguchi, Y., Yamawaki, Y., Gao, J., Harada, K., Takeuchi, H., ... & Kanematsu, T. (2017). Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling. Scientific Reports, 7.
  36. Bugge, M., Bergstrom, B., Eide, O. K., Solli, H., Kjønstad, I. F., Stenvik, J., ... & Nilsen, N. J. (2017). Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness. Journal of Biological Chemistry, jbc-M117.
  37. Murgueitio, M. S., Ebner, S., Hörtnagl, P., Rakers, C., Bruckner, R., Henneke, P., ... & Santos-Sierra, S. (2017). Enhanced immunostimulatory activity of in silico discovered agonists of Toll-like receptor 2 (TLR2). Biochimica et Biophysica Acta (BBA)-General Subjects.
  38. Gu, S., Sachleben, J. R., Boughter, C. T., Nawrocka, W. I., Borowska, M. T., Tarrasch, J. T., ... & Adams, E. J. (2017). Phosphoantigen-induced conformational change of butyrophilin 3A1 (BTN3A1) and its implication on Vγ9Vδ2 T cell activation. Proceedings of the National Academy of Sciences, 201707547.
  39. Ferioli, S., Zierler, S., Zaißerer, J., Schredelseker, J., Gudermann, T., & Chubanov, V. (2017). TRPM6 and TRPM7 differentially contribute to the relief of heteromeric TRPM6/7 channels from inhibition by cytosolic Mg2+ and Mg· ATP. Scientific Reports, 7.
  40. Sugimoto, R., Watanabe, H., Ikegami, K., Enoki, Y., Imafuku, T., Sakaguchi, Y., ... & Tanaka, M. (2017). Down-regulation of ABCG2, a urate exporter, by parathyroid hormone enhances urate accumulation in secondary hyperparathyroidism. Kidney international, 91(3), 658-670.
  41. Lee, A., Choi, K. M., Jung, W. B., Jeong, H., Kim, G. Y., Lee, J. H., ... & Yoo, H. S. (2017). Enhancement of Glucose Uptake by Meso-Dihydroguaiaretic Acid through GLUT4 Up-Regulation in 3T3-L1 Adipocytes. Molecules, 22(9), 1423.
  42. Thornton, P., Sevalle, J., Deery, M. J., Fraser, G., Zhou, Y., Ståhl, S., ... & Nicol, L. S. (2017). TREM2 shedding by cleavage at the H157‐S158 bond is accelerated for the Alzheimer's disease‐associated H157Y variant. EMBO Molecular Medicine, e201707673.
  43. Toyoki, D., Shibata, S., Kuribayashi-Okuma, E., Xu, N., Ishizawa, K., Hosoyamada, M., & Uchida, S. (2017). Insulin stimulates uric acid reabsorption via regulating urate transporter 1 and ATP-binding cassette subfamily G member 2. American Journal of Physiology-Renal Physiology, 313(3), F826-F834.
  44. Thorwald, M., Rodriguez, R., Lee, A., Martinez, B., Peti-Peterdi, J., Nakano, D., ... & Ortiz, R. M. (2017). Angiotensin receptor blockade improves cardiac mitochondrial activity in response to an acute glucose load in obese insulin resistant rats. Redox Biology.
  45. Liu, J., Liu, W., Yang, L., Wu, Q., Zhang, H., Fang, A., ... & Tang, F. (2017). The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding. Cell Stem Cell.
  46. Yokoi, K., Hisamatsu, Y., Naito, K. and Aoki, S. (2017), Design, Synthesis, and Anticancer Activities of Cyclometalated Tris(2-phenylpyridine)iridium(III) Complexes with Cationic Peptides at the 4′-Position of the 2-Phenylpyridine Ligand. Eur. J. Inorg. Chem. doi: 10.1002/ejic.201700846
  47. Dong, J., Zhou, Q., Wei, Z., Yan, S., Sun, F., & Cai, X. (2017). Protein kinase A mediates scopolamine-induced mTOR activation and an antidepressant response. Journal of Affective Disorders.
  48. Liu, H., Xu, X., Han, T., Yan, L., Cheng, L., Qin, Y., & Chen, Z. J. (2017). A novel homozygous mutation in the FSHR gene is causative for primary ovarian insufficiency. Fertility and Sterility.
  49. Chapman, K. T., White, M. P., Wang, X., Park, M., Stadler, G. K., Lowe, R. D & Radel, P. A. (2017). U.S. Patent Application No. 15/406,289.
  50. Park, B. N., Lee, S. J., Roh, J. H., Lee, K. H., An, Y. S., & Yoon, J. K. (2017). Radiolabeled Anti- Adenosine Triphosphate Synthase Monoclonal Antibody as a Theragnostic Agent Targeting Angiogenesis. Molecular Imaging, 16, 1536012117737399.
  51. Tian, Y., Heng, D., Xu, K., Liu, W., Weng, X., Hu, X., & Zhang, C. (2017). cGMP/PKG-I pathway-mediated GLUT1/4 regulation by NO (nitric oxide) in female rat granulosa cells. Endocrinology.
  52. Kameyama, N., Munetoshi, A.N.D.O., Ogawa, S. and Okada, K., Kyowa Hakko Kirin Co Ltd, 2017. Antibody which specifically binds to human crth2. U.S. Patent Application 15/342,534.
  53. Claffey, Kevin P., Charan Devarakonda, and Daniel Kita. "ANTIBODY AND ANTIGEN-BINDING FRAGMENT COMPOSITIONS TARGETING CELL SURFACE ANTIGENS IN TUMORS AND METHODS OF USE THEREOF." U.S. Patent Application 15/637,402, filed January 4, 2018.
  54. Li, C., Du, S., Tian, M., Wang, Y., Bai, J., Tan, P., ... & Li, Y. (2018). The host restriction factor IFITM3 Inhibits Vaccinia Virus Infection. Frontiers in Immunology, 9, 228.
  55. Long, L., Pang, X. X., Lei, F., Zhang, J. S., Wang, W., Liao, L. D., ... & Wang, L. D. (2018). SLC52A3 expression is activated by NF-κB p65/Rel-B and serves as a prognostic biomarker in esophageal cancer. Cellular and Molecular Life Sciences, 1-19.
  56. Yu, Y., Zhao, Y., Teng, F., Li, J., Guan, Y., Xu, J., ... & Chen, L. Berberine Improves Cognitive Deficiency and Muscular Dysfunction Via Activation of the AMPK/SIRT1/PGC-1A Pathway in Skeletal Muscle from Naturally Aging Rats. The journal of nutrition, health & aging, 1-8.
  57. Song, X., Shen, Q., Fan, L., Yu, Q., Jia, X., Sun, Y., ... & Kang, J. (2018). Dehydroepiandrosterone-induced activation of mTORC1 and inhibition of autophagy contribute to skeletal muscle insulin resistance in a mouse model of polycystic ovary syndrome. Oncotarget, 9(15), 11905.
  58. Yin, X., Feng, L., Ma, D., Yin, P., Wang, X., Hou, S., ... & Feng, J. (2018). Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone. Journal of Neuroinflammation, 15(1), 97.
  59. Zhang, X. L., Ding, H. H., Xu, T., Liu, M., Ma, C., Wu, S. L., ... & Xin, W. J. (2018). Palmitoylation of δ-catenin promotes kinesin-mediated membrane trafficking of Nav1. 6 in sensory neurons to promote neuropathic pain. Sci. Signal., 11(523), eaar4394.
  60. Yamauchi, T., Doi, S., Nakashima, A., Doi, T., Sohara, E., Uchida, S., & Masaki, T. (2018). Na+-Cl− cotransporter-mediated chloride uptake contributes to hypertension and renal damage in aldosterone-infused rats. American Journal of Physiology-Renal Physiology.
  61. Kameyama, Naoya, A. N. D. O. Munetoshi, Shinya Ogawa, and Kazuki Okada. "Antibody which specifically binds to human CRTH2." U.S. Patent 9,938,349, issued April 10, 2018.
  62. Choi, K. M., & Yoo, H. S. (2018). 3, 3′‐Diindolylmethane Enhances Glucose Uptake Through Activation of Insulin Signaling in 3T3‐L1 Adipocytes. Obesity.
  63. Liu, Q., Zhao, Y., Zheng, L., Zhu, X., Cui, L., & Cao, Y. (2018). The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malaria. Infection and immunity, IAI-00929.
  64. Wu, Y., Liu, T., Yuan, Y., & Zhang, Z. (2018). Gene expression profile of TLR7 signaling pathway in the liver of rhesus rotavirus-induced murine biliary atresia. Biochemical and biophysical research communications.
  65. Komatsu, W., Kishi, H., Yagasaki, K., & Ohhira, S. (2018). Urolithin A attenuates pro-inflammatory mediator production by suppressing PI3-K/Akt/NF-κB and JNK/AP-1 signaling pathways in lipopolysaccharide-stimulated RAW264 macrophages: Possible involvement of NADPH oxidase-derived reactive oxygen species.
  66. European Journal of Pharmacology.
  67. Uchida, Y., Sumiya, T., Tachikawa, M., Yamakawa, T., Murata, S., Yagi, Y., ... & Couraud, P. O. (2018). Involvement of Claudin-11 in Disruption of Blood-Brain,-Spinal Cord, and-Arachnoid Barriers in Multiple Sclerosis. Molecular Neurobiology, 1-18.
  68. Shibata, S., Ishizawa, K., Wang, Q., Xu, N., Fujita, T., Uchida, S., & Lifton, R. P. (2018). ULK1 Phosphorylates and Regulates Mineralocorticoid Receptor. Cell Reports, 24(3), 569-576.
  69. Wang, J., Wang, Z., Liu, R., Shuai, L., Wang, X., Luo, J., ... & He, X. (2018). Metabotropic glutamate receptor subtype 2 is a cellular receptor for rabies virus. PLOS Pathogens, 14(7), e1007189.
  70. Liu Q, Zhao Y, Zheng L, Zhu X, Cui L, Cao Y. 2018. The glycosylphosphatidylinositol transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malaria. Infect Immun 86:e00929-17.
  71. Liu, D. Y., Chi, T. Y., Ji, X. F., Liu, P., Qi, X. X., Zhu, L., ... & Zou, L. B. (2018). Sigma-1 receptor activation alleviates blood-brain barrier dysfunction in vascular dementia mice. Experimental neurology, 308, 90-99.
  72. Kairupan, T. S., Cheng, K. C., Asakawa, A., Amitani, H., Yagi, T., Ataka, K., ... & Inui, A. (2018). Rubiscolin-6 activates opioid receptors to enhance glucose uptake in skeletal muscle. Journal of Food and Drug Analysis7He,
  73. X., Du, C., Zou, Y., Long, Y., Huang, C., Chen, F., ... & Zhou, X. (2018). Downregulation of MicroRNA-4463 Attenuates High-Glucose-and Hypoxia-Induced Endothelial Cell Injury by Targeting PNUTS. Cellular Physiology and Biochemistry49(5), 2073-2087.
  74. Hirohama, D., Ayuzawa, N., Ueda, K., Nishimoto, M., Kawarazaki, W., Watanabe, A., ... & Fujita, T. (2018). Aldosterone Is Essential for Angiotensin II-Induced Upregulation of Pendrin. Journal of the American Society of Nephrology29(1), 57-68.
  75. Thorwald, M. A. (2018). The Regulation of Cardiac Nrf2 During an Acute Glucose Challenge and the Impact on Mitochondrial Function During Insulin Resistance and Type II Diabetes Mellitus (Doctoral dissertation, UC Merced).
  76. Xu et al., (2018) Phosphorylation-Mediated IFN-gR2 MembraneTranslocation Is Required to Activate Macrophage Innate Response. Cell 175, 1–16.
  77. Alam, M. B., An, H., Ra, J. S., Lim, J. Y., Lee, S. H., Yoo, C. Y., & Lee, S. H. (2018). Gossypol from Cottonseeds Ameliorates Glucose Uptake by Mimicking Insulin Signaling and Improves Glucose Homeostasis in Mice with Streptozotocin-Induced Diabetes. Oxidative Medicine and Cellular Longevity2018.
  78. Hakuno, D., Kimura, M., Ito, S., Satoh, J., Nakashima, Y., Horie, T., ... & Nishi, H. (2018). Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction. Scientific reports, 8(1), 16749.
  79. Wang, D., Ba, H., Li, C., Zhao, Q., & Li, C. (2018). Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC–MS/MS. International journal of molecular sciences, 19(11), 3477.
  80. Kuroda, H., Tachikawa, M., Yagi, Y., Umetsu, M., Nurdin, A., Miyauchi, E., ... & Terasaki, T. (2018). Cluster of Differentiation 46 is the Major Receptor in Human Blood-Brain Barrier Endothelial Cells for Uptake of Exosomes Derived from Brain-Metastatic Melanoma Cells (SK-Mel-28). Molecular pharmaceutics.
  81. Kataoka, Y., Ohshio, Y., Teramoto, K., Igarashi, T., Asai, T., & Hanaoka, J. Hypoxia‑induced galectin‑3 enhances RhoA function to activate the motility of tumor cells in non‑small cell lung cancer. Oncology Reports.
  82. Hoshi, Y., Uchida, Y., Tachikawa, M., Ohtsuki, S., Couraud, P. O., Suzuki, T., & Terasaki, T. (2019). Oxidative stress-induced activation of Abl and Src kinases rapidly induces P-glycoprotein internalization via phosphorylation of caveolin-1 on tyrosine-14, decreasing cortisol efflux at the blood–brain barrier. Journal of Cerebral Blood Flow & Metabolism, 0271678X18822801.
  83. Zhang, N., Zhao, H., & Zhang, L. (2019). Fatty acid synthase promotes the palmitoylation of Chikungunya virus nsP1. Journal of virology, 93(3), e01747-18.
  84. Nakamura, H., Nagamine, A., Yashima, H., Araki, T., & Yamamoto, K. (2019). Exploration of Proteins Involved in Acquisition of Resistance to Cetuximab. Indonesian Journal of Pharmaceutics, 1(1).
  85. Chen, X. Y., Ren, H. H., Wang, D., Chen, Y., Qu, C. J., Pan, Z. H., ... & Li, D. F. (2019). Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells. Oxidative Medicine and Cellular Longevity, 2019.
  86. Ishizawa, K., Wang, Q., Li, J., Yamazaki, O., Tamura, Y., Fujigaki, Y., ... & Shibata, S. (2019). Calcineurin dephosphorylates Kelch-like 3, reversing phosphorylation by angiotensin II and regulating renal electrolyte handling. Proceedings of the National Academy of Sciences, 201817281.
  87. Nemoto, Y., Kumagai, T., Ishizawa, K., Miura, Y., Shiraishi, T., Morimoto, C., ... & Fujigaki, Y. (2019). Phosphate binding by sucroferric oxyhydroxide ameliorates renal injury in the remnant kidney model. Scientific Reports, 9(1), 1732.
  88. i, Y., Yang, T., Yao, Q., Li, S., Fang, E., Li, Y., ... & Li, W. (2019). Metformin prevents colonic barrier dysfunction by inhibiting mast cell activation in maternal separation‐induced IBS‐like rats. Neurogastroenterology & Motility.
  89. Peng, W., Tong, C., Li, L., Huang, C., Ran, Y., Chen, X., ... & Luo, X. (2019). Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia. The FASEB Journal, fj-201802058RR.
  90. Zhong, W., Xu, M., Li, C., Zhu, B., Cao, X., Li, D., ... & Pan, G. (2019). ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCβ3 Catalysis: Targeting It Eradicates Leukemia Stem Cells. Cell Reports, 26(8), 2166-2177.
  91. Zhang, S. H., Liu, D. X., Wang, L., Li, Y. H., Wang, Y. H., Zhang, H., ... & Li, B. (2019). A CASPR1–ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. Journal of Biological Chemistry, jbc-RA118.
  92. Nagamine, A., Araki, T., Nagano, D., Miyazaki, M., & Yamamoto, K. L‑Lactate dehydrogenase B may be a predictive marker for sensitivity to anti‑EGFR monoclonal antibodies in colorectal cancer cell lines. Oncology Letters.
  93. Essandoh, K., Deng, S., Wang, X., Jiang, M., Mu, X., Peng, J., ... & Fan, G. C. (2019). Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R. The FASEB Journal, fj-201802338RR.

  94. Ishizawa, K., Wang, Q., Li, J., Xu, N., Nemoto, Y., Morimoto, C., ... & Fujita, T. (2019). Inhibition of Sodium Glucose Cotransporter 2 Attenuates the Dysregulation of Kelch-Like 3 and NaCl Cotransporter in Obese Diabetic Mice. Journal of the American Society of Nephrology, ASN-2018070703.

  95. Van Krieken, R., Mehta, N., Wang, T., Zheng, M., Li, R., Gao, B., ... & Austin, R. C. (2019). Cell surface expression of 78 kDa glucose regulated protein (GRP78) mediates diabetic nephropathy. Journal of Biological Chemistry, jbc-RA118.
  96. Li, C., Wang, X., Cheng, F., Du, X., Yan, J., Zhai, C., ... & Wang, Q. (2019). Geniposide protects against hypoxia/reperfusion-induced blood-brain barrier impairment by increasing tight junction protein expression and decreasing inflammation, oxidative stress, and apoptosis in an in vitro system. European Journal of Pharmacology.
  97. Ni, H. M., Chao, X., Kaseff, J., Deng, F., Wang, S., Shi, Y. H., ... & Jaeschke, H. (2019). Receptor-Interacting Serine/Threonine Kinase 3 (RIP3)–Mixed Lineage Kinase Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers. The American Journal of Pathology.
  98. Sheng, J., Shen, L., Sun, L., Zhang, X., Cui, R., & Wang, L. (2019). Inhibition of PI3K/mTOR increased the sensitivity of hepatocellular carcinoma cells to cisplatin via interference with mitochondrial‐lysosomal crosstalk. Cell Proliferation, e12609.
  99. Ghosh, M., Lo, R., Ivic, I., Aguilera, B., Qendro, V., Devarakonda, C., & Shapiro, L. H. (2019). CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, β1 integrin recycling, and cell migration. , 12(579), eaav5938.
  100. Liu, W., Wang, X., Wang, S., Ba, X., Xu, T., Wang, X., & Zeng, X. RhoGDI2 positively regulates the Rho GTPases activation in response to the β2 outside‐in signaling in T cells adhesion and migration on ICAM‐1. Journal of Leukocyte Biology.

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