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Minute™ Detergent-Free Nuclei Isolation Kit (20 Preps)

Minute™ Detergent-Free Nuclei Isolation Kit (20 Preps)

Catalog Number: NI-024

  • $238.00


Manual & Protocol | Material Safety Data Sheets (MSDS)

Minute™ Detergent-Free Nuclei Isolation Kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Unlike many other methods where detergents are used to lyse the cell membranes, intact nuclei can be isolated from the samples using this patented technology in less than 20 minutes without using any detergent or a tissue homogenizer. One major problem associated with the use of detergent is the tendency of nuclei aggregation, which makes it difficult to be separated into a single nucleus. To overcome this shortcoming, we have developed this detergent-free nuclei isolation kit. The sample is first incubated with buffer A that sensitizes the cells for mechanical disruption. The cell suspension quickly passes through a specially designed tilter cartridge. The cell membranes are ruptured when passing through the filter leaving intact native nuclei to flow through. The nuclei are separated from other small cell debris by low-speed centrifugation using a proprietary buffer B. The native and intact isolated nuclei can be used for a variety of applications, which include but are not limited to FACS analysis, single nucleus RNA sequencing, chromosome immunoprecipitation (ChIP), immunofluorescence staining, cell cycle analysis and/or apoptosis research.

Kit includes:

Items

Quantity

Buffer A

15 ml

Buffer B

30 ml

Protein Extraction Filter Cartridges

20 units

Collection Tubes

20 units

Pestles

2 units


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