Extraction of nuclear matrix protein from cultured cells or tissues – Invent Biotechnologies Inc.

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Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps)

Catalog Number: NM-033

  • $599.00
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Manual & Protocol | MSDS

Nucleoplasm, also referred to as karyoplasm/nucleus sap, consists of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate membrane-bound proteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein extraction kit is designed to separate cellular protein into three fractions: cytosolic, nuclear matrix and water-insoluble fractions (mainly envelope and nucleic acid-associated proteins) using a patented spin-column-based technology. The buffers used are detergent and EDTA-free. The whole protocol can be completed in less than 1 hour. The proteins extracted are in their native state and can be used in many downstream applications such as gel retardation, transcription factor analysis, apoptosis and protein trafficking studies.  



Buffer A

30 ml

Buffer B

5 ml

Protein Extraction Filter Cartridges

20 units

Plastic Rods

2 units

Collection Tubes

20 units


  1. Luo, Q., Wu, X., Zhao, P., Nan, Y., Chang, W., Zhu, X., ... & Liu, Z. (2021). OTUD1 Activates Caspase‐Independent and Caspase‐Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Advanced Science, 2002874.

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