Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)

Manual & Protocol | MSDS 

Lysosomes, found in eukaryotic cells, are spherical vesicles responsible for the removal of cellular waste. These organelles contain digestive enzymes that break down excess or worn-out organelles, food particles, and engulfed viruses and bacteria. Lysosomes are relatively large, ranging from 0.1 to 1.2 µm. In cell research, isolating lysosomes is a pivotal step for investigating processes such as autophagy, protein degradation, and recycling.

Traditional lysosome isolation methods, which originated in the 1970s, face challenges such as requiring substantial starting material and being time-consuming, often leading to significant cross-contamination. However, this kit offers a groundbreaking solution, using patented spin-column-based technology characterized by its simplicity, speed, and efficiency. With this kit, native lysosomes can be enriched without a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in less than 90 minutes, utilizing only 20-30 milligrams of the starting material.

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