Total Protein Extraction Kit – Invent Biotechnologies Inc.

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Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps)

Catalog number: SD-001/SN-002

  • $365.00



Manual & ProtocolMaterial Safety Data Sheets (MSDS)


MinuteTM total protein extraction kit for animal cultured cells and tissues is the most advanced next-generation tool for super-fast protein extraction without altered protein profile usually associated with solution-based procedures. More and more evidence has shown that the most commonly used RIPA buffer can cause unpredictable protein loss, resulting in questionable data interpretation. This problem is fully resolved using the patented spin column-based technologies. Coupled with stronger lysis buffers, the spin column makes protein extraction super easy and efficient. The extraction volume can be as low as 20 µl – a helpful feature when starting material is a limiting factor. This kit provides denaturing and native cell lysis buffers so users can select according to specific applications. Total proteins can be extracted from cultured cells/tissues in 1-8 min with high yield (2-8 mg/ml).

-RIPA Buffer - The Potential Troubles

-Pitfalls of Protein Extraction by RIPA

-A Technical Breakthrough for Protein Sample Preparation

Selected references related to protein loss by RIPA buffer:

  1. Bai, B., and Laiho, M. (2012) Proteomics. 12:3044-3048
  2. Mukhopadhyay, C. et al. (2016) PNAS 5:8228-8237
  3. Li, Q. (2016) Biotechniques. 61:327
  4. Ngoka, L. CM. (2008) Proteome Science. 6:30

    Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, goldfish muscle and 5, mouse liver.    Kit includes:

    Items

    Quantity

    Denaturing Cell Lysis Buffer

    25 ml

    Native Cell Lysis Buffer

    25 ml

    Protein Extraction Filter Cartridges

    50 units

    Collection Tubes with Caps

    50 units

    Plastic Rods

    2 units


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