Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)

Manual & Protocol | MSDS 

Lysosomes, found in eukaryotic cells, are spherical vesicles responsible for the removal of cellular waste. These organelles contain digestive enzymes crucial for breaking down excess or worn-out organelles, food particles, as well as viruses and bacteria that have been engulfed. Lysosomes exhibit a relatively large size, ranging from 0.1 to 1.2 micrometers. In the realm of cell research, the initial isolation of lysosomes is a pivotal step when investigating processes like autophagy, protein degradation, and recycling.

Traditional methods for lysosome isolation, which originated in the 1970s, are associated with challenges such as the need for substantial starting material and a time-consuming procedure that often leads to significant cross-contaminations. However, a groundbreaking solution is offered by this kit, utilizing patented spin-column-based technology that is characterized by its simplicity, speed, and efficiency. With this kit, native lysosomes can be notably enriched without the requirement of a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in less than 90 minutes, utilizing only 20-30 milligrams of the starting material.

Compare with Other Lysosome Isolation Kits