Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adip – Invent Biotechnologies Inc.

Have a question? Ask our scientists!


Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps)

Catalog Number: AT-022

  • $495.00
Shipping calculated at checkout.

Manual & ProtocolMaterial Safety Data Sheets (MSDS)

Adipose tissue, especially white adipose tissue (WAT), has been recognized as an essential endocrine and inflammation organ in addition to its energy storage function. Analyses of proteins from adipose tissues are increasingly critical for understanding many physiological/pathological conditions. However, isolation of WAT and brown adipose tissue (BAT) is technically challenging due to their high lipid and low protein contents. The water-oil emulsion present in a biological sample is notoriously difficult to separate. We have developed a novel technology to address this issue. A porous filter with unique surface property and pre-defined pore size and thickness coupling with a specially formulated detergent-free extraction buffer is employed to rapidly and effectively separate water-oil emulsion derived from adipose tissue homogenate. The extraction buffer has a lower freezing point than oil in adipose tissues, and the aqueous phase can be quickly separated from the oil phase by passing the tissue homogenate through the filter. The total proteins isolated are the unbiased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg/ml) compared to traditional methods.



A. SDS-PAGE (10%) profiles of total protein extracted from different adipose tissues. Lane 1, porcine WAT; Lane 2, chicken BAT; Lane 3, rat WAT.

B. Western blottings of extracted proteins from rat WAT. Proteins were separated in 8-16% gradient SDS-PAGE and probed with following cellular protein marker antibodies:

Anti-Na/K ATPase alpha1, a plasma membrane marker (Upstate, clone 464.6), anti-lamin B1, a nuclear envelope marker (ab16048, abcam Cambridge, MA), anti-ubiquinol-cytochrome C reductase core protein (abcam, ab 96333) and GAPDH, a cytosolic marker (Sigma). The specific protein bands were visualized by a substrate Opti-4CN (Bio-RAD).

 Kit includes:



Buffer A (Extraction Buffer)

15 ml

Buffer B (10 X Denaturing Buffer)

1.5 ml

Buffer C (10 X Non-Denaturing Buffer)

1.5 ml

1.5 ml Microfuge Tubes

20 units

Pestles for 1.5 ml Tubes

2 units

Filter Cartridge with Collection Tubes

20 units

Protein Extraction Powder

2 grams

  1. Wang, N., Liu, Y., Ma, Y., & Wen, D. (2017). High-intensity interval versus moderate-intensity continuous training: Superior metabolic benefits in diet-induced obesity mice. Life Sciences.
  2. Zheng, Q., Lin, J., Huang, J., Zhang, H., Zhang, R., Zhang, X., ... & Song, R. (2017). Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity. Proceedings of the National Academy of Sciences, 201707853.
  3. Invent, B. I. (2017). High Efficiency Protein Extraction from Adipose Tissues. BioTechniques, 63(5), 235
  4. Battson, M. L., Lee, D. M., Jarrell, D. K., Hou, S., Ecton, K. E., Weir, T. L., & Gentile, C. L. (2017). Suppression of Gut Dysbiosis Reverses Western Diet-Induced Vascular Dysfunction. American Journal of Physiology-Endocrinology and Metabolism.
  5. Chen, G., Li, Q., Luo, Y., Liu, T., Zhou, S., Pan, E., & Peng, L. (2018). Effect of Notoginsenoside R1 on autologous adipose graft in rats. Molecular Medicine Reports.
  6. Chen, T. X., Cheng, X. Y., Wang, Y., & Yin, W. (2018). Toosendanin inhibits adipogenesis by activating Wnt/β-catenin signaling. Scientific Reports, 8(1), 4626.
  7. Wang, N., Liu, Y., Ma, Y., & Wen, D. (2018). Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. The Journal of Nutritional Biochemistry.
  8. Du, W., Piek, A., Schouten, E. M., van de Kolk, C. W., Mueller, C., Mebazaa, A., ... & Silljé, H. H. Plasma levels of heart failure biomarkers are primarily a reflection of extracardiac production. Theranostics, Vol. 8 4155-4169
  9. Hann, M. (2018). Anti-inflammatory Activity of Isomaltodextrin in a C57BL/6NCrl Mouse Model with Lipopolysaccharide-induced Low-grade Chronic Inflammation (Doctoral dissertation).
  10. Liu, L., Wang, N., Ma, Y., Liu, Y., & Wen, D. (2018). Saponins from Boussingaultia gracilis prevent obesity and related metabolic impairments in diet-induced obese mice. Food & Function.
  11. Ott, R., Melchior, K., Stupin, J. H., Ziska, T., Schellong, K., Henrich, W., ... & Plagemann, A. (2018). Reduced insulin receptor expression and altered DNA methylation in fat tissues and blood of women with GDM and offspring. The Journal of Clinical Endocrinology & Metabolism.
  12. Yue, S. J., Liu, J., Wang, A. T., Meng, X. T., Yang, Z. R., Peng, C., ... & Yan, D. (2018). Berberine alleviates insulin resistance by reducing peripheral branched-chain amino acids. American Journal of Physiology-Endocrinology and Metabolism.
  13. Shah, A. P., Johnson, M. D., Fu, X., Boersma, G. J., Shah, M., Wolfgang, M. J., ... & Baraban, J. M. (2019). Deletion of translin (Tsn) induces robust adiposity and hepatic steatosis without impairing glucose tolerance. International Journal of Obesity, 1.
  14. Li, M., Wang, M., Liu, Y., Huang, S., Yi, X., Yin, C., ... & Xiao, Y. (2019). TNF‐α Upregulates IKKε Expression via the Lin28B/let‐7a Pathway to Induce Catecholamine Resistance in Adipocytes. Obesity.
  15. Jahandideh, F., de Campos Zani, S. C., Son, M., Proctor, S. D., Davidge, S. T., Chan, C. B., & Wu, J. (2019). Egg white hydrolysate enhances insulin sensitivity in high fat diet induced insulin resistant rats via AKT activation. British Journal of Nutrition, 1-25.
  16. Zhang, F., Li, D., Wu, Q., Sun, J., Guan, W., Hou, Y., ... & Wang, J. (2019). Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows. Journal of Animal Science and Biotechnology, 10(1), 38.
  17. Wang, N., Ma, Y., Liu, Z., Liu, L., Yang, K., Wei, Y., ... & Wen, D. (2019). Hydroxytyrosol prevents PM2. 5-induced adiposity and insulin resistance by restraining oxidative stress related NF-κB pathway and modulation of gut microbiota in a murine model. Free Radical Biology and Medicine, 141, 393-407. Zani, S. (2019).
  18. Exploratory study of egg white hydrolysate mechanisms of action in insulin sensitive tissues of high fat diet-induced insulin resistant rats. https://doi.org/10.7939/r3-vpcv-w240
  19. Vianello, E., Dozio, E., Bandera, F., Froldi, M., Micaglio, E., Lamont, J., ... & Schmitz, G. (2020). Correlative study on impaired prostaglandin E2 regulation in EAT and maladaptive cardiac remodeling via EPAC2 and ST2 signaling in overweight CVD subjects. International Journal of Molecular Sciences, 21, 520.
  20. Fu, X., Shah, A. P., Li, Z., Li, M., Tamashiro, K. L., & Baraban, J. M. (2020). Genetic inactivation of the translin/trax microRNA-degrading enzyme phenocopies the robust adiposity induced by Translin (Tsn) deletion. bioRxiv
  21. An, Y. A., & Scherer, P. E. Mouse Adipose Tissue Protein Extraction.
  22. Tu, J., Zhu, S., Li, B., Xu, G., Luo, X., Jiang, L., ... & Chen, C. (2020). Gegen Qinlian Decoction Coordinately Regulates PPARγ and PPARα to Improve Glucose and Lipid Homeostasis in Diabetic Rats and Insulin Resistance 3T3-L1 Adipocytes. Frontiers in Pharmacology, 11.
  23. Li, X., Lu, H. Y., Jiang, X. W., Yang, Y., Xing, B., Yao, D., ... & Zhao, Q. C. (2020).          Cinnamomum cassia extract promotes thermogenesis during exposure to cold via activation of brown adipose tissue. Journal of Ethnopharmacology, 113413.
  24. Oldoni, F., Cheng, H., Banfi, S., Gusarova, V., Cohen, J. C., & Hobbs, H. H. (2020). ANGPTL8 has both endocrine and autocrine effects on substrate utilization. JCI insight.
  25. .Jiang, X. W., Lu, H. Y., Xu, Z. H., Zhang, Y. S., & Zhao, Q. C. (2020). Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia. Fitoterapia, 104824.

  26. Cheng, C., Xue, F., Sui, W., Meng, L., Xie, L., Zhang, C., ... & Zhang, Y. (2021). Deletion of natriuretic peptide receptor C alleviates adipose tissue inflammation in hypercholesterolemic Apolipoprotein E knockout mice. Journal of Cellular and Molecular Medicine.

  27. Li, Y., Wang, H., Liao, Y., Yan, Q., Ouyang, Z., Zhao, Y., ... & Lai, L. (2021). CREBRF p. Arg457Gln Promotes Obesity and Improves Insulin Sensitivity by Promoting Preadipocyte Differentiation and Reducing Oxidative Metabolism in Gene-modified Pig Models

  28.  Wang, Y., Jiang, H. F., Liu, B. B., Chen, L. L., Wang, Y., Liu, X. Y., ... & Wu, X. F. (2021). Brown Adipose Tissue Activation Is Involved in Atherosclerosis of ApoE−/− Mice Induced by Chronic Intermittent Hypoxia. Frontiers in Cardiovascular Medicine, 8.

  29. Hill, J. L., McIver, K. B., Katzer, K., & Foster, M. T. (2022). Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue. Methods and Protocols, 5(2), 34.

  30. Liu, Z., Chen, T., Zhang, S., Yang, T., Gong, Y., Deng, H. W., ... & Chen, Y. (2022). Discovery and functional assessment of a novel adipocyte population driven by intracellular Wnt/β-catenin signaling in mammals. eLife, 11, e77740.
  31. de Campos Zani, S. C., Son, M., Bhullar, K. S., Chan, C. B., & Wu, J. (2022). IRW (Isoleucine–Arginine–Tryptophan) Improves Glucose Tolerance in High Fat Diet Fed C57BL/6 Mice via Activation of Insulin Signaling and AMPK Pathways in Skeletal Muscle. Biomedicines, 10(6), 1235.
  32. Cao, S., Pan, Y., Tang, J., Terker, A. S., Arroyo Ornelas, J. P., Jin, G. N., ... & Zhang, M. Z. (2022). EGFR-mediated activation of adipose tissue macrophages promotes obesity and insulin resistance. Nature Communications, 13(1), 1-16.

  33. Li, X., Zhang, X., Shen, Z., Chen, Z., Wang, H., & Zhang, X. (2022). GnRH receptor mediates lipid storage in female adipocytes via AMPK pathway. Int J Med Sci, 19(9), 1442-1450.

  34. .Kristina Schlicht; Juliane Brandes; Isabelle Zobel; Nathalie Rohmann; Corinna Geisler; et al2022.Dipeptidylpeptidase (DPP)-4 inhibitor therapy increases circulating levels of anti-inflammatory soluble frizzle receptor protein (sFRP)-5 which is decreased in severe COVID-19 disease.Diabetologie Und Stoffwechsel
  35. de Campos Zani, S. C., Wang, R., Veida-Silva, H., Clugston, R. D., Yue, J. T., Mori, M. A., ... & Chan, C. B. (2023). An Egg White-Derived Peptide Enhances Systemic Insulin Sensitivity and Modulates Markers of Non-Alcoholic Fatty Liver Disease in Obese, Insulin Resistant Mice. Metabolites13(2), 174.
  36. Li, D., Chen, B., Yuan, S., Zhang, M., Wang, X., Liu, R., ... & Chen, X. (2023). Analysis of Adipose Tissue Cytokine Profile in a Chronic Kidney Disease Model with Insulin Resistance.

  37. Hunstiger, M., Johannsen, M. M., & Oliver, S. R. (2023). Non-shivering thermogenesis is differentially regulated during the hibernation season in Arctic ground squirrels. Frontiers in Physiology, 14.
  38. Cheng, C., Zhang, J., Li, X., Xue, F., Cao, L., Meng, L., ... & Zhang, C. (2023). NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice. Signal Transduction and Targeted Therapy, 8(1), 290.

  39. Gehle, P. F., Satzinger, S., Willenborg, S., & Eming, S. A. (2023). Isolation of dermal adipocytes from mouse skin for biochemical analysis. STAR protocols, 4(4), 102765.

  40. de Campos Zani, S. C., Berg, E., Jiang, X., Knox, A., Ackroyd, E., Getachew, A., ... & Chan, C. B. (2024). IRW improves diet-induced non-alcoholic fatty liver disease by reducing steatosis associated with increased capacity for oxidative phosphorylation. Journal of Functional Foods, 112, 105976.

  41. Lu, M., Yu, Z., Yang, X., An, L., Jing, X., Xu, T., ... & Yu, Z. (2024). Remodelling the inguinal adipose sensory system to switch on the furnace: Electroacupuncture stimulation induces brown adipose thermogenesis. Diabetes, Obesity and Metabolism.
  42. Jia, W., Giwa, R., Moley, J. R., Smith, G. I., Petersen, M. C., Field, R. L., ... & Brestoff, J. R. (2024). MXRA8 promotes adipose tissue whitening to drive obesity. bioRxiv, 2024-01.

  43. .  Smagris, E., Shihanian, L. M., Mintah, I. J., Bigdelou, P., Livson, Y., Brown, H., ... & Gusarova, V. (2024). Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse. PLoS genetics, 20(3), e1011179.

  44. Vermehren-Schmaedick, A., Joshi, S., Wagoner, W., Norgard, M. A., Packwood, W., Diba, P., ... & Luoh, S. W. (2024). Grb7 ablation in mice improved glycemic control, enhanced insulin signaling and increased abdominal fat mass in females. Endocrinology, bqae045.

  45. Xie X, Liu Y, Yang Q, Ma X, Lu Y, Hu Y, Zhang G, Ke L, Tong Z, Liu Y, Xue J, Lu G, Li W. Adipose Triglyceride Lipase-Mediated Adipocyte Lipolysis Exacerbates Acute Pancreatitis Severity in Mouse Models and Patients. Am J Pathol. 2024 May 3:S0002-9440(24)00165-2. doi: 10.1016/j.ajpath.2024.03.014. Epub ahead of print. PMID: 38705384.
  46. Wei, W., Lyu, X., Markhard, A. L., Fu, S., Mardjuki, R. E., Cavanagh, P. E., ... & Long, J. Z. (2024). A PTER-dependent pathway of taurine metabolism linked to energy balance. bioRxiv.

We Also Recommend