Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps) – Invent Biotechnologies Inc.

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Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)

Catalog Number: LY-034

  • $595.00
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Manual & Protocol | MSDS 

Lysosomes, found in eukaryotic cells, are spherical vesicles responsible for the removal of cellular waste. These organelles contain digestive enzymes crucial for breaking down excess or worn-out organelles, food particles, as well as viruses and bacteria that have been engulfed. Lysosomes exhibit a relatively large size, ranging from 0.1 to 1.2 micrometers. In the realm of cell research, the initial isolation of lysosomes is a pivotal step when investigating processes like autophagy, protein degradation, and recycling.

Traditional methods for lysosome isolation, which originated in the 1970s, are associated with challenges such as the need for substantial starting material and a time-consuming procedure that often leads to significant cross-contaminations. However, a groundbreaking solution is offered by this kit, utilizing patented spin-column-based technology that is characterized by its simplicity, speed, and efficiency. With this kit, native lysosomes can be notably enriched without the requirement of a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in less than 90 minutes, utilizing only 20-30 milligrams of the starting material.

Compare with Other Lysosome Isolation Kits





Buffer A

15 ml

Buffer B

2 ml

Protein Extraction Filter Cartridges

20 units

Plastic Rods

2 units

Collection Tubes

20 units


1. Ravodina, A. M., Badgeley, M. A., Rajagopalan, S., Fedyukina, D. V., & Maiseyeu, A. (2020). Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays. ACS Omega.

2. Kim, J. W., Mahiddine, F. Y., & Kim, G. A. (2020). Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). International Journal of Molecular Sciences, 21(23), 8963.

3. Casey, C. A., Donohue Jr, T. M., Kubik, J. L., Kumar, V., Naldrett, M. J., Woods, N. T., ... & Thomes, P. G. (2021). Lipid droplet membrane proteome remodeling parallels ethanol-induced hepatic steatosis and its resolution. Journal of Lipid Research, 100049.

4.  Jing, Z., Gao, J., Li, J., Niu, F., Tian, L., Nan, P., ... & Zhao, X. (2021). Acetylation-induced PCK isoenzyme transition promotes metabolic adaption of liver cancer to systemic therapy. Cancer Letters.

5. Gordevicius, J., Li, P., Marshall, L. L., Killinger, B. A., Lang, S., Ensink, E., ... & Labrie, V. (2021). Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease. Nature Communications, 12(1), 1-18.

6. Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database2022.

7.  Cao, X., Meng, P., Shao, Y., Yan, G., Yao, J., Zhou, X., ... & Lu, H. (2022). Nascent Glycoproteome Reveals That N-Linked Glycosylation Inhibitor-1 Suppresses Expression of Glycosylated Lysosome-Associated Membrane Protein-2. Frontiers in Molecular Biosciences9.

8.  Tao, L., Qing, Y., Cui, Y., Shi, D., Liu, W., Chen, L., ... & Zhang, L. (2022). Lysosomal membrane permeabilization mediated apoptosis involve in perphenazine-induced hepatotoxicity in vitro and in vivo. Toxicology Letters.

9.  Tsujimoto, K., Jo, T., Nagira, D., Konaka, H., Park, J. H., Yoshimura, S. I., ... & Kumanogoh, A. (2022). The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6. The EMBO Journal, e111389.

10.  Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

11.  Qing, Y., Guo, Y., Zhao, Q., Hu, P., Li, H., Yu, X., ... & Hui, H. (2023). Targeting lysosomal HSP70 induces acid sphingomyelinase‐mediated disturbance of lipid metabolism and leads to cell death in T cell malignancies. Clinical and Translational Medicine, 13(3), e1229.

12.  Li, Y., Fan, Z., Jia, Q., Ma, H., Wu, Y., Guo, X., ... & Zhang, X. (2023). Chaperone-mediated autophagy (CMA) alleviates cognitive impairment by reducing neuronal death in sepsis-associated encephalopathy (SAE). Experimental Neurology, 114417.

13.  Ren, G., Ding, Y. W., Wang, L. L., & Jiang, J. D. (2023). Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1. Frontiers in Pharmacology, 14.

14.  Secker, C., Motzny, A. Y., Kostova, S., Buntru, A., Helmecke, L., Reus, L., ... & Wanker, E. E. (2023). The polyphenol EGCG directly targets intracellular amyloid‐β aggregates and promotes their lysosomal degradation. Journal of Neurochemistry.

15.  Lin, C. P., Wu, S. H., Lin, T. Y., Chu, C. H., Lo, L. W., Kuo, C. C., ... & Huang, D. M. Lysosomal-Targeted Doxorubicin Delivery Using Rbc-Derived Vesicles to Overcome Drug-Resistant Cancer Through Mitochondrial-Dependent Cell Death. Available at SSRN 4492777.

16.  Chen, L., Ma, Y., Ma, X., Liu, L., Jv, X., Li, A., ... & Xie, J. (2023). TFEB regulates cellular labile iron and prevents ferroptosis in a TfR1-dependent manner. Free Radical Biology and Medicine.

17.  Morishige, J. I., Yoshioka, K., Nakata, H., Ishimaru, K., Nagata, N., Tanaka, T., ... & Ando, H. (2023). Sphingosine Kinase 1 is Involved in Triglyceride Breakdown by Maintaining Lysosomal Integrity in Brown Adipocytes. Journal of Lipid Research, 100450.

18.  He, L., Chen, J., Deng, P., Huang, S., Liu, P., Wang, C., ... & Lin, C. (2023). Lysosomal cyst (e) ine storage potentiates tolerance to oxidative stress in cancer cells. Molecular Cell.

19. Wang, Z., He, W., Li, C., Chen, Y., Li, Z., Jiao, Y., ... & Lan, Y. (2023). The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. Journal of Virology, e01338-23.

20.  Jiang Y, Chen X, Ye X, Wen C, Xu T, Yu C, Ning W, Wang G, Xiang X, Liu X, Wang Y, Chen Y, Liu X, Shi C, Liu C, Yuan Q, Chen Y, Zhang T, Luo W, Xia N. A Dual-domain Engineered Antibody for Efficient HBV Suppression and Immune Responses Restoration. Adv Sci (Weinh). 2024 Feb 11:e2305316. doi: 10.1002/advs.202305316. Epub ahead of print. PMID: 38342604.

21.  Wang, S., Li, B., Cai, Z., Hugo, C., Li, J., Sun, Y., ... & Yassine, H. (2024). Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD.


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