Minute™ High Fidelity ER Isolation Kit for Liver Tissue (20 Preps)

Manual & Protocol | MSDS

Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or ultracentrifugation. The whole protocol can be done in about 2 hours.