Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)

Manual & Protocol | MSDS 

Lysosomes are spherical vesicles in eukaryotic cells that are responsible for waste removal. The digestive enzymes contained in lysosomes play a vital role in digesting excess or worn-out organelles, food particles and engulfed viruses or bacteria. Lysosomes are relatively large organelles ranging in size from 0.1 to 1.2 um. The ability to isolate lysosomes is an important first step in studies of autophagy, protein degradation and protein recycling in a cell. Traditional methods for isolating lysosomes are based on density-gradient ultracentrifugation. A large amount of starting material is required and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for lysosome isolation are based on methods developed in the 1970s. Unlike any other lysosome isolation kit on the market, our kit employs a patented spin-column-based technology that is simple, rapid and efficient. The amount of starting cells/tissues required is much smaller than that of traditional methods. This kit can significantly enrich lysosomes from cultured cells or tissues without using a Dounce homogenizer and ultracentrifugation. The whole protocol can be done in less than 1.5 hours.

Compare with Other Lysosome Isolation Kits

1. Ravodina, A. M., Badgeley, M. A., Rajagopalan, S., Fedyukina, D. V., & Maiseyeu, A. (2020). Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays. ACS Omega.

2. Kim, J. W., Mahiddine, F. Y., & Kim, G. A. (2020). Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). International Journal of Molecular Sciences, 21(23), 8963.

3. Casey, C. A., Donohue Jr, T. M., Kubik, J. L., Kumar, V., Naldrett, M. J., Woods, N. T., ... & Thomes, P. G. (2021). Lipid droplet membrane proteome remodeling parallels ethanol-induced hepatic steatosis and its resolution. Journal of Lipid Research, 100049.

4.  Jing, Z., Gao, J., Li, J., Niu, F., Tian, L., Nan, P., ... & Zhao, X. (2021). Acetylation-induced PCK isoenzyme transition promotes metabolic adaption of liver cancer to systemic therapy. Cancer Letters.

5. Gordevicius, J., Li, P., Marshall, L. L., Killinger, B. A., Lang, S., Ensink, E., ... & Labrie, V. (2021). Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease. Nature Communications, 12(1), 1-18.

6. Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.

7.  Cao, X., Meng, P., Shao, Y., Yan, G., Yao, J., Zhou, X., ... & Lu, H. (2022). Nascent Glycoproteome Reveals That N-Linked Glycosylation Inhibitor-1 Suppresses Expression of Glycosylated Lysosome-Associated Membrane Protein-2. Frontiers in Molecular Biosciences, 9.

8.  Tao, L., Qing, Y., Cui, Y., Shi, D., Liu, W., Chen, L., ... & Zhang, L. (2022). Lysosomal membrane permeabilization mediated apoptosis involve in perphenazine-induced hepatotoxicity in vitro and in vivo. Toxicology Letters.

9.  Tsujimoto, K., Jo, T., Nagira, D., Konaka, H., Park, J. H., Yoshimura, S. I., ... & Kumanogoh, A. (2022). The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6. The EMBO Journal, e111389.

10.  Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

11.  Qing, Y., Guo, Y., Zhao, Q., Hu, P., Li, H., Yu, X., ... & Hui, H. (2023). Targeting lysosomal HSP70 induces acid sphingomyelinase‐mediated disturbance of lipid metabolism and leads to cell death in T cell malignancies. Clinical and Translational Medicine, 13(3), e1229.

12.  Li, Y., Fan, Z., Jia, Q., Ma, H., Wu, Y., Guo, X., ... & Zhang, X. (2023). Chaperone-mediated autophagy (CMA) alleviates cognitive impairment by reducing neuronal death in sepsis-associated encephalopathy (SAE). Experimental Neurology, 114417.

13.  Ren, G., Ding, Y. W., Wang, L. L., & Jiang, J. D. (2023). Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1. Frontiers in Pharmacology, 14.

14.  Secker, C., Motzny, A. Y., Kostova, S., Buntru, A., Helmecke, L., Reus, L., ... & Wanker, E. E. (2023). The polyphenol EGCG directly targets intracellular amyloid‐β aggregates and promotes their lysosomal degradation. Journal of Neurochemistry.

15.  Lin, C. P., Wu, S. H., Lin, T. Y., Chu, C. H., Lo, L. W., Kuo, C. C., ... & Huang, D. M. Lysosomal-Targeted Doxorubicin Delivery Using Rbc-Derived Vesicles to Overcome Drug-Resistant Cancer Through Mitochondrial-Dependent Cell Death. Available at SSRN 4492777.

16.  Chen, L., Ma, Y., Ma, X., Liu, L., Jv, X., Li, A., ... & Xie, J. (2023). TFEB regulates cellular labile iron and prevents ferroptosis in a TfR1-dependent manner. Free Radical Biology and Medicine.