Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues comprises optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to protein extraction filter cartridges, the protein isolation procedure is simple and provides a high yield. The process can be completed in less than 30 minutes. Unlike many commercial mitochondria preparation kits, this kit offers a wide range of starting cells and isolated mitochondria. The buffers are detergent and EDTA-free. A Dounce homogenizer or a tissue blender is not needed.

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute™ Mitochondria Isolation Kit. 

 A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3, 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues, respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues, respectively.

 B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048, a nuclear envelope marker protein. abcam, Cambridge, MA). 

 C. Densitometry measurement of mitochondrial marker signals.

Kit includes:

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4. Zhang, L., Liu, J., Zhou, F., Wang, W., & Chen, N. (2018). PGC-1α ameliorates kidney fibrosis in mice with diabetic kidney disease through an antioxidative mechanism. Molecular medicine reports.
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6. Shen, L., Sun, B., Sheng, J., Yu, S., Li, Y., Xu, H., ... & Sun, L. (2018). PGC1α promotes cisplatin resistance in human ovarian carcinoma cells through upregulation of mitochondrial biogenesis. International Journal of Oncology.
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10. Xue, Y. N., Yu, B. B., Li, J. L., Guo, R., Zhang, L. C., Sun, L. K., ... & Li, Y. (2018). Zinc and p53 Disrupt Mitochondrial Binding of HK2 by Phosphorylating VDAC1. Experimental Cell Research.
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20. Hu, X., Xia, M., Wang, J., Yu, H., Chai, J., Zhang, Z., ... & Sun, L. (2020). Dual PI3K/mTOR inhibitor PKI-402 suppresses the growth of ovarian cancer cells by degradation of Mcl-1 through autophagy. Biomedicine & Pharmacotherapy, 129, 110397.
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22. Deng, X., Lin, N., Fu, J., Xu, L., Luo, H., Jin, Y., ... & Su, J. (2020). The Nrf2/PGC1α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer. Oxidative Medicine and Cellular Longevity, 2020.
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29. Wu, X., Li, J., Wang, S., Jiang, L., Sun, X., Liu, X., ... & Yang, G. (2021). 2-Undecanone Protects against Fine Particle-Induced Kidney Inflammation via Inducing Mitophagy. Journal of Agricultural and Food Chemistry.
30. Zhao, L., Liu, D., Ma, W., Gu, H., Wei, X., Luo, W., & Yuan, Z. (2021). Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta. Frontiers in Cell and Developmental Biology, 9, 1025.
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33. Duan, C., Kuang, L., Hong, C., Xiang, X., Liu, J., Li, Q., ... & Li, T. (2021). Mitochondrial Drp1 recognizes and induces excessive mPTP opening after hypoxia through BAX-PiC and LRRK2-HK2. Cell Death & Disease, 12(11), 1-10.
34. Li, T., Zhao, G., Zhang, T., Zhang, Z., Chen, X., Song, J., ... & Weng, C. (2021). African Swine Fever Virus pE199L Induces Mitochondrial-Dependent Apoptosis. Viruses, 13(11), 2240.
35. Meng, H., Sun, L. K., Su, J., Yan, W. Y., Jin, Y., Luo, X., ... & Wang, H. L. (2021). Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis. Human Cell, 1-20.
36. Li, X., Hou, P., Ma, W., Wang, X., Wang, H., Yu, Z., ... & He, H. (2021). SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy. Cellular & Molecular Immunology, 1-12.

37. Yan, X., Qu, X., Liu, B., Zhao, Y., Xu, L., Yu, S., ... & SU, J. (2021). Autophagy-induced HDAC6 activity during hypoxia regulates mitochondrial energy metabolism through the β-catenin/COUP-TFII axis in hepatocellular carcinoma cells. Frontiers in Oncology, 4275. 

38. Xiao, L., Hu, Q., Peng, Y., Zheng, K., Zhang, T., Yang, L., ... & Liu, Y. (2021). TRAP1 suppresses oral squamous cell carcinoma progression by reducing oxidative phosphorylation metabolism of Cancer-associated fibroblasts. BMC cancer, 21(1), 1-14.
39. Cheng, M., Yu, H., Kong, Q., Wang, B., Shen, L., Dong, D., & Sun, L. (2022). The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. International Journal of Molecular Sciences, 23(3), 1320.

40. Liu, J., Li, L., Xie, P., Zhao, X., Shi, D., Zhang, Y., ... & Li, T. (2022). Sevoflurane induced neurotoxicity in neonatal mice links to a GSK3β/Drp1-dependent mitochondrial fission and apoptosis. Free Radical Biology and Medicine.

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42. He, X. T., Li, X., Zhang, M., Tian, B. M., Sun, L. J., Bi, C. S., ... & Chen, F. M. (2022). Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. Biomaterials, 121439.
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59. Wu, K., Huang, C., Zheng, W., Wu, Y., Huang, Q., Lin, M., ... & Liu, L. (2023). Activation of mitophagy improves cognitive dysfunction in diabetic mice with recurrent non-severe hypoglycemia. Molecular and Cellular Endocrinology, 112109.

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