Minute™ Chloroplast Isolation Kit (50 Preps)

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Minute™ Chloroplast Isolation Kit (50 Preps)

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Catalog Number: CP-011

$375.00

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References (24)

Manual & Protocol | Material Safety Data Sheets (MSDS)

Invent Biotechnologies Minute^TMchloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.). Due to the use of filter cartridges with pre-defined pore size and thickness, intact chloroplasts can be isolated from 50-200 mg fresh plant tissues in less than 5 min. Unlike many other methods that require 1-10+ gram tissues for chloroplast isolation, this kit can quickly obtain 1 X 10⁶ to 1 X 10⁷ intact chloroplasts (>90% intact) from fresh plant leaves.

Kit includes:

Items	Quantity
Buffer A (with 5 grams dissociation beads)	25 ml
Buffer B	25 ml
Protein Extraction Filter Cartridges	50 units
Collection Tubes with Caps	50 units
Plastic Rods	2 units

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Hu, D., Li, Y., Jin, W., Gong, H., He, Q., & Li, Y. (2017). Identification and Characterization of a Plastidic Adenine Nucleotide Uniporter (OsBT1-3) Required for Chloroplast Development in the Early Leaf Stage of Rice. Scientific Reports, 7, 41355.
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