High Efficiency Protein Extraction from Adipose Tissues

Introduction

Proteomic research using adipose tissues are increasingly common. It is relatively easy to extract total proteins from most cell types and tissues however it is much more difficult to extract high quality total proteins from adipose tissues. A common feature shared by adipose tissues is their high lipid and low protein content. In average, the protein content accounts for less than 2% of total cellular mass, making it technically very challenging to isolate high concentration total proteins from adipose tissues [1]. Here we report a high efficiency total protein extraction method from adipose tissues.