Blog – Invent Biotechnologies Inc.
中文

Blog

High Efficiency Protein Extraction from Adipose Tissues

Posted by Ainsley Shea on

Introduction Proteomic research using adipose tissues are increasingly common. It is relatively easy to extract total proteins from most cell types and tissues however it is much more difficult to extract high quality total proteins from adipose tissues. A common feature shared by adipose tissues is their high lipid and low protein content. In average, the protein content accounts for less than 2% of total cellular mass, making it technically very challenging to isolate high concentration total proteins from adipose tissues [1]. Here we report a high efficiency total protein extraction method from adipose tissues.

Read more →

Protein Extraction from Adipose Tissues--- A Tough Challenge

Posted by Ainsley Shea on

Protein Extraction from Adipose Tissues--- A Tough Challenge Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions.  Therefore, understanding the development and function of adipocytes are essential to understanding metabolic homeostasis under physiological and pathological conditions. In recent years, proteomic research using adipose tissues are increasingly common [1]. It is relatively easy to extract total proteins from most cell types and tissues however it is much more difficult to extract high quality total proteins from adipose tissues. As shown below, there are basically three types of adipocytes (Brown, Brite and white). A...

Read more →

Tips for Detecting Phosphoproteins by Western Blot

Posted by Invent Biotechnologies Inc. on

Tips for Detecting Phosphoproteins by Western Blot Protein phosphorylation is very important for protein post-translational modification that controls many cellular processes including metabolism, transcriptional and translation regulation, degradation of proteins, cellular signaling and communication, proliferation, differentiation, and cell survival [1]. Approximately 35% of human proteins are phosphorylated. Phosphoproteins are low in abundance, and, therefore, are, sometimes, challenging to detect and characterize by Western blot and other methods. Western blot is the most common method for detecting and quantifying phosphorylated proteins. Western for phosphoprotein is not really different from a regular Western blot but following factors need pay attention to in...

Read more →

RIPA Buffer- The Potential Troubles

Posted by Invent Biotechnologies Inc. on

RIPA Buffer- The Potential TroublesInvent Biotechnologies Inc.Radioimmunoprecipitation assay buffer (RIPA buffer) is the most commonly used buffer for total protein extraction from vertebrate cultured cells and tissues [1] Extracted proteins can be used for a variety of downstream applications such as SDS-PAGE, Western blotting, nucleic acid binding, immunoprecipitation, co-precipitation, ELISA, enzymatic activity assay and affinity-based protein purifications. Due to the large heterogeneity of proteins and interfering contaminants, it is a great challenge to simultaneous release and solubilize all proteins in a given sample. Integration of proteins into membranes and the formation of complexes with other proteins or nucleic acids in...

Read more →

A Technical Breakthrough for Protein Sample Preparation

Posted by Ainsley Shea on

A Technical Breakthrough for Protein Sample Preparation

A Technical Breakthrough for Protein Sample Preparation (Solution-based vs. Spin Column-Based Technologies) Sample preparation is usually the first step for almost all protein work. The quality of such preparation is critical to successful protein analysis. The quality of protein sample dictates the quality of the experimental results. There is an increasing demand for high quality, small scale protein preparations for analytical purposes. As protein analysis has become more and more complex, the demand for adequate sample preparation techniques has likewise grown. Protein sample preparation is increasingly critical for people who work on proteomics, functional genomics, clinical studies, differential expression, protein...

Read more →