Catalog number: SD-001/SN-002
Manual & Protocol | Material Safety Data Sheets (MSDS)
MinuteTM total protein extraction kit for animal cultured cells and tissues is the most advanced next-generation tool for super-fast protein extraction without altered protein profile usually associated with solution-based procedures. More and more evidence has shown that the most commonly used RIPA buffer can cause unpredictable protein loss, resulting in questionable data interpretation. This problem is fully resolved using the patented spin column-based technologies. Coupled with stronger lysis buffers, the spin column makes protein extraction super easy and efficient. The extraction volume can be as low as 20 µl – a helpful feature when starting material is a limiting factor. This kit provides denaturing and native cell lysis buffers so users can select according to specific applications. Total proteins can be extracted from cultured cells/tissues in 1-8 min with high yield (2-8 mg/ml).
-RIPA Buffer - The Potential Troubles
-Pitfalls of Protein Extraction by RIPA
-A Technical Breakthrough for Protein Sample Preparation
Selected references related to protein loss by RIPA buffer:
Items
Quantity
Denaturing Cell Lysis Buffer
25 ml
Native Cell Lysis Buffer
Protein Extraction Filter Cartridges
50 units
Collection Tubes with Caps
Plastic Rods
2 units
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Minute™ Yeast/Fungus Nuclear and Cytoplasmic Protein Extraction Kit (20 preps)
Minute™ Plasma Membrane Isolation Kit for Mammalian Red Blood Cells (20 preps)
Minute™ Water-soluble and Water-Insoluble Protein Fractionation Kit (20 preps)
Minute™ Thylakoid Enrichment and Chloroplast Fractionation Kit (20 preps)
Minute™ Single Nuclei Isolation Kit for Plant Tissues (20 preps)