Catloge # WS-056
Manual & Protocol | Material Safety Data Sheets (MSDS)
Researchers studying protein localization and trafficking now have a convenient tool: the MinuteTM Water-soluble and Water-Insoluble Protein Fractionation Kit for cells/tissues. This unique product allows for the rapid and efficient separation of these two key fractions from cells/tissues, microorganisms and plant tissues, providing enriched and easily detectable protein samples for downstream applications like western blotting, ELISA and immunoprecipitation. Water soluble fraction contains mainly cytosolic proteins and water-insoluble fraction contains mainly insoluble membranous structures such as plasma membrane, nuclear membrane and organelles. The kit offers researchers a novel and simple approach for understanding protein function and movement within the cellular landscape. If a protein target is known to be localized in water-soluble or water-insoluble fraction, the kit will facilitate detection, isolation and purification of the target protein by enrich corresponding fractions.
Fig. 1. Protein profiles of water-insoluble (WI) and water-soluble (WS) fractions from HEK293 cells, yeast (S. cerevisiae) and plant (Broccoli leaf). Proteins were separated in 10% SDS-PAGE and stained with Coomassie blue.
Fig. 2. Western blotting of fractionated frozen mouse liver. Lanes 1, 2 and 3 are total liver lysate, water-insoluble and water-soluable fraction, respectively. A. Ponceau red stained blot.
B. Western blot with anti-Na/K-ATPase (plasma membrane marker), Lamin B1 (nuclear envelope marker) and GAPDH (cytosolic marker).
Kit includes:
Items
Quantity
Buffer A
15 ml
Buffer B
25 ml
Buffer C
1 ml
Buffer D
Buffer N
1.5 ml Microfuge Tubes
20 units
Pestles for 1.5 ml Tubes
2 units
Protein Extraction Powder
2 grams
Minute™ Yeast/Fungus Nuclear and Cytoplasmic Protein Extraction Kit (20 preps)
Minute™ Plasma Membrane Isolation Kit for Mammalian Red Blood Cells (20 preps)
Minute™ Thylakoid Enrichment and Chloroplast Fractionation Kit (20 preps)
Minute™ Single Nuclei Isolation Kit for Plant Tissues (20 preps)
Minute™ Chloroplast Isolation kit for Mucilaginous Plants (20 preps)
