Catalog #: ED-028
Manual & Protocol | Material Safety Data Sheets (MSDS)
Early endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues.
Kit includes:
Items
Quantity
Buffer A
15 ml
Buffer B
Plastic Rods
2 units
Filter Cartridge
20 units
Tissue Dissociation Beads
2.5 grams
Collection Tubes
Minute™ Yeast/Fungus Nuclear and Cytoplasmic Protein Extraction Kit (20 preps)
Minute™ Plasma Membrane Isolation Kit for Mammalian Red Blood Cells (20 preps)
Minute™ Water-soluble and Water-Insoluble Protein Fractionation Kit (20 preps)
Minute™ Thylakoid Enrichment and Chloroplast Fractionation Kit (20 preps)
Minute™ Single Nuclei Isolation Kit for Plant Tissues (20 preps)
