Catalog Number: FE-025
Manual & Protocol | Material Safety Data Sheets (MSDS)
Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potentially rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin-mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at a high temperature with or without an optional sonication step. These methods can be effective but are tedious and time-consuming (3-4 hours). Minute™ Protein Extraction Kit for Fixed and Embedded Tissues provides a simple and rapid way to extract protein from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less than one hour with a protein yield of 2-3 mg/ml.
Kit includes:
Items
Quantity
Buffer A
30 ml
Buffer B
10 ml
1.5 ml Microfuge Tubes
20 units
Pestles
2 units
1. Gnanaguru, G., Wagschal, A., Oh, J., Saez-Torres, K. L., Li, T., Temel, R. E., ... & D’Amore, P. A. (2021). Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration. Molecular Therapy.
2. Obi, E. N., Tellock, D. A., Thomas, G. J., & Veenstra, T. D. (2023). Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics. Biomolecules, 13(1), 96.
3. Moriyama, R., Nakamura, S., Mitsui, I., Sugiyama, M., Fukui, H., Fukui, H., ... & Suzuki, J. (2023). Expression of SARS-CoV-2 entry molecules ACE2, NRP1, TMPRSS2, and FURIN in the reproductive tissues of male macaques.
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