Catalog Number: GO-037
Manual & Protocol | MSDS
The Golgi apparatus, also known as the Golgi complex or Golgi body, consists of a series of flattened stacked pouches called cisternae. This organelle plays a crucial role in eukaryotic cells by facilitating the transportation, modification, and packaging of proteins and lipids into vesicles for delivery to specific locations. The quantity and distribution of Golgi vary significantly across different cell and tissue types. Obtaining a highly enriched Golgi fraction is a crucial initial step in the study of its function and interactions with other organelles.
Traditional methods for isolating the Golgi apparatus rely on density gradient ultracentrifugation, which demands a substantial amount of starting material and can be laborious and time-consuming. In contrast, the Minute™ kit distinguishes itself from other Golgi isolation kits by employing patented spin-column-based technology. This approach is both straightforward and rapid, requiring only a small amount of starting material. With this kit, native Golgi can be preferentially enriched through precipitation, eliminating the need for a Dounce homogenizer and ultracentrifugation. It enables the isolation of two sub-Golgi fractions: the Golgi apparatus and secretory vesicles of the Golgi.
Compare with Other Golgi Apparatus Enrichment Kits
Kit Components:
Quantity
Buffer A
20 ml
Buffer B
8 ml
Buffer C
2 ml
Plastic Rods
2 units
Filter Cartridge
20 units
Collection Tubes
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