Comparison of Major Lysosome Isolation Kits – Invent Biotechnologies Inc.

Comparison of Major Lysosome Isolation Kits

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Lysosomes are membranous vesicles that are rich in hydrolytic enzymes capable of breaking down proteins, nuclei acids, carbohydrates and other cellular components. There are several commercial kits available for lysosome isolation. However, by mechanism of isolation, there are only two types of kits: traditional density gradient centrifugation-based and novel spin column-based.

 

Brand/manufacturer Brand# 1 Brand# 2 Brand# 3 Invent Biotech
Product Name Lysosome Isolation Kit from Tissue and Cultured Cells Lysosome Isolation Kit Lysosome Enrichment Kit for Tissue and Cultured cells MinuteTM Lysosome Isolation Kit
Mechanism of Isolation Density gradient centrifugation Density gradient centrifugation Density gradient centrifugation Spin column-based -precipitation
Average sample size Tissue (100 mg) Cells (20 million) Tissue (4 g) Cells (3000 million) Tissue (125 mg) Cells (125 mg) Tissue (25 mg) Cells (25 million)
Number of buffers and reagents used 5 7 3 2
Buffer preparation prior to use Required Required Required Not required
Homogenizer Required Required Required Not required
Density gradient formation Required Required Required Not required
Ultracentrifugation Required Required Required Not required
Purity of Isolated Lysosomes Enriched lysosomes Enriched lysosomes Enriched lysosomes Enriched lysosomes
Approximate Protocol Time About 3h 5-12h About 3h About 1.2h