Comparison of Major Lysosome Isolation Kits
Download as a pdfLysosomes are membranous vesicles that are rich in hydrolytic enzymes capable of breaking down proteins, nuclei acids, carbohydrates and other cellular components. There are several commercial kits available for lysosome isolation. However, by mechanism of isolation, there are only two types of kits: traditional density gradient centrifugation-based and novel spin column-based.
| Brand/manufacturer | Brand# 1 | Brand# 2 | Brand# 3 | Invent Biotech |
|---|---|---|---|---|
| Product Name | Lysosome Isolation Kit from Tissue and Cultured Cells | Lysosome Isolation Kit | Lysosome Enrichment Kit for Tissue and Cultured cells | MinuteTM Lysosome Isolation Kit |
| Mechanism of Isolation | Density gradient centrifugation | Density gradient centrifugation | Density gradient centrifugation | Spin column-based -precipitation |
| Average sample size | Tissue (100 mg) Cells (20 million) | Tissue (4 g) Cells (3000 million) | Tissue (125 mg) Cells (125 mg) | Tissue (25 mg) Cells (25 million) |
| Number of buffers and reagents used | 5 | 7 | 3 | 2 |
| Buffer preparation prior to use | Required | Required | Required | Not required |
| Homogenizer | Required | Required | Required | Not required |
| Density gradient formation | Required | Required | Required | Not required |
| Ultracentrifugation | Required | Required | Required | Not required |
| Purity of Isolated Lysosomes | Enriched lysosomes | Enriched lysosomes | Enriched lysosomes | Enriched lysosomes |
| Approximate Protocol Time | About 3h | 5-12h | About 3h | About 1.2h |