Comparison of Major Lipid Raft Isolation Kits – Invent Biotechnologies Inc.

Comparison of Major Lipid Raft Isolation Kits

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Lipid rafts are important subcellular structures rich in cholesterol and sphingolipid and signaling proteins. Lipid rafts play a critical role in cellular transport and signal transduction. Traditional method of lipid raft isolation is tedious and time consuming. There are limited numbers of commercial kits for isolation of this important structure.

 

Brand/manufacturer Brand# 1 Invent Biotech
Product Name Caveolae/Rafts Isolation Kit MinuteTM Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues
Mechanism of Isolation Ultracentrifugation flotation Spin column-based - floatation with a table top centrifuge
Average sample size Cells (20-50 million) Tissue (35 mg) Cells (20-30 million)
Number of buffers and reagents used 6 3
Buffer preparation prior to use Required Not required
Homogenizer Required Not required
Density gradient formation Not Required Not required
Ultracentrifugation Not Required Not required
Purity of Isolated Lipid Rafts Total Detergent Resistant lipid Rafts Total Detergent Resistant lipid Rafts
Approximate Protocol Time >5 h 1h