Comparison of Major Lipid Raft Isolation Kits
Download as a pdfLipid rafts are important subcellular structures rich in cholesterol and sphingolipid and signaling proteins. Lipid rafts play a critical role in cellular transport and signal transduction. Traditional method of lipid raft isolation is tedious and time consuming. There are limited numbers of commercial kits for isolation of this important structure.
| Brand/manufacturer | Brand# 1 | Invent Biotech |
|---|---|---|
| Product Name | Caveolae/Rafts Isolation Kit | MinuteTM Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues |
| Mechanism of Isolation | Ultracentrifugation flotation | Spin column-based - floatation with a table top centrifuge |
| Average sample size | Cells (20-50 million) | Tissue (35 mg) Cells (20-30 million) |
| Number of buffers and reagents used | 6 | 3 |
| Buffer preparation prior to use | Required | Not required |
| Homogenizer | Required | Not required |
| Density gradient formation | Not Required | Not required |
| Ultracentrifugation | Not Required | Not required |
| Purity of Isolated Lipid Rafts | Total Detergent Resistant lipid Rafts | Total Detergent Resistant lipid Rafts |
| Approximate Protocol Time | >5 h | 1h |