Comparison of Major Golgi Apparatus Isolation Kits
Download as a pdfThe Golgi apparatus is consisted of a series offlattened membrane vesicles.The major function of Golgi is to modify proteins for transportation.Modified proteins are transported out of Golgi in coated vesicles that bud off the trans‐cellular membrane. It plays a critical role in protein synthesis and modification. There are limited selections of commercial kits for isolation of this important organelle.
| Brand/manufacturer | Brand# 1 | Invent Biotech |
|---|---|---|
| Product Name | Golgi Isolation Kit | nuteTM Golgi Apparatus Enrichment Kit |
| Mechanism of Isolation | Density gradient centrifugation | Spin column-based -precipitation |
| Average sample size | Tissue (5 g) | Tissue (35 mg) Cells (30 million) |
| Number of buffers and reagents used | 3 | 4 |
| Buffer preparation prior to use | Required | Not required |
| Homogenizer | Required | Not required |
| Density gradient formation | Not Required | Not required |
| Ultracentrifugation | Not Required | Not required |
| Purity of Isolated Golgi | Enriched Golgi | Enriched cis-Golgi and secretory vesicles of trans-Golgi |
| Approximate Protocol Time | 3.5h | 2h |