Comparison of Major ER Isolation Kits – Invent Biotechnologies Inc.

Comparison of Major ER Isolation Kits

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The endoplasmic reticulum (ER) is a membranous labyrinth extending throughout the cell. The ER is a major organelle and accounts for about 1/2 of total cellular membrane. It plays a critical role in protein synthesis and modification. There are a few commercial kits available for ER isolation with different mechanisms of actions.

 

Brand/manufacturer Brand# 1 Brand# 2 Invent Biotech
Product Name ER Enrichment Kit Endoplasmic Reticulum Isolation Kit MinuteTM ER Enrichment Kit
Mechanism of Isolation Differential centrifugation and precipitation Density gradient centrifugation Spin column-based -precipitation
Average sample size Tissue (500 mg) Tissue (3-7 g) Cells (2000 million) Tissue (35 mg) Cells (30 million)
Number of buffers and reagents used 5 4 4
Buffer preparation prior to use Required Required Not required
Homogenizer Required Required Not required
Density gradient formation Not Required Required Not required
Ultracentrifugation Not Required Required Not required
Purity of Isolated ER Enriched ER Enriched ER Enriched ER
Approximate Protocol Time 1-2h 1.5-5h 1.5h