Comparison of Major ER Isolation Kits
The endoplasmic reticulum (ER) is a membranous labyrinth extending throughout the cell. The ER is a major organelle and accounts for about 1/2 of total cellular membrane. It plays a critical role in protein synthesis and modification. There are a few commercial kits available for ER isolation with different mechanisms of actions.
Brand/manufacturer | Brand# 1 | Brand# 2 | Invent Biotech |
---|---|---|---|
Product Name | ER Enrichment Kit | Endoplasmic Reticulum Isolation Kit | MinuteTM ER Enrichment Kit |
Mechanism of Isolation | Differential centrifugation and precipitation | Density gradient centrifugation | Spin column-based -precipitation |
Average sample size | Tissue (500 mg) | Tissue (3-7 g) Cells (2000 million) | Tissue (35 mg) Cells (30 million) |
Number of buffers and reagents used | 5 | 4 | 4 |
Buffer preparation prior to use | Required | Required | Not required |
Homogenizer | Required | Required | Not required |
Density gradient formation | Not Required | Required | Not required |
Ultracentrifugation | Not Required | Required | Not required |
Purity of Isolated ER | Enriched ER | Enriched ER | Enriched ER |
Approximate Protocol Time | 1-2h | 1.5-5h | 1.5h |