Catalog Number: SC-012
Manual & Protocol | Material Safety Data Sheets (MSDS)
Minute™ Single Cell Isolation Kit is composed of optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes.
The kit is designed to rapidly isolate single cells from fresh animal tissues with high cell viability as well as isolate nuclei from tissues briefly fixed with formaldehyde for chromosome immunoprecipitation (ChIP). The buffers don’t contain any proteinases and EDTA that may have adverse effects on cell surface markers. Single-cell suspension can be obtained from fresh tissues in less than 10 minutes with the use of filter cartridges that have a pre-defined pore size and specially formulated buffer system. The efficacy of the kit is tissue type dependent and is especially effective for lymphoid tissues such as spleen, thymus and lymph node. For fresh tissues with a high amount of connective tissue content, the efficacy is variable. This kit is not recommended for fresh tissues such as muscle, intestine and lungs. For fixed tissues, tissue types are less critical.
For storage and transportation of isolated single nuclei, the following buffer is recommended:
WA-014: Minute™ Anti-Clumping Nuclei Storage Buffer
Buffer A (for non-fixed tissues)
Buffer B (for fixed tissues)
Cell Isolation Filter Cartridges
Collection Tubes with Caps
Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)