Minute™ Plant Microsomal Membrane Extraction Kit (50 Preps)

Manual & Protocol | MSDS

Isolation of microsomal membranes from plant tissues is a common laboratory procedure. The microsomal fraction of plant cell lysate is the focus of interest in many plant research projects. The microsomal fraction is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes, and other components of the membrane system. The traditional method for microsomal fraction isolation involves a so-called differential pelleting protocol, where a series of centrifugation steps are required to obtain various membrane fractions. The traditional protocol for microsomal fraction isolation requires a large amount of starting material and employs tedious ultracentrifugation steps. MM-018 offers a simple, rapid, and user-friendly approach for microsomal membrane extraction using a small amount of starting material (200 mg). Water-soluble cytosolic proteins are removed during the procedure, and the water-insoluble microsomal fraction, especially the plasma membrane fraction, is extracted with optimized buffers in a table-top microcentrifuge. The procedure is simple and rapid, and no special instruments are required. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg/sample. 

Kit includes:

References (4)

1. Kim, S., Choi, Y., Kwon, C., & Yun, H. S. (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. Journal of integrative plant biology.
2. Yang, Z., Yang, J., Wang, Y., Wang, F., Mao, W., He, Q., ... & Mao, C. (2020). PROTEIN PHOSPOHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice. The Plant Cel
3. Yu, F., Cao, X., Liu, G., Wang, Q., Xia, R., Zhang, X., & Xie, Q. (2020). ESCRT-I Component VPS23A Is Targeted by E3 Ubiquitin Ligase XBAT35 for Proteasome-Mediated Degradation in Modulating ABA Signaling. Molecular Plant, 13(11), 1556-1569.
4. Kato, T., Morita, R., Ootsuka, S., Wakabayashi, Y., Aoki, N., & Horibata, A. Evaluation of alleles at OsAGPS2, OsAGPL2, and OsSUT1 related to grain filling in rice in a common genetic background. Crop Science.
5. Hou, L. Y., Lehmann, M., & Geigenberger, P. (2021). Thioredoxins o1 and h2 Show Different Subcellular Localizations and Redox-Active Functions, but Cooperatively Affect NADPH Redox Poise and Photosynthetic Performance in Fluctuating Light.
6. Chiu, C. Y., Tsai, C. D., Wang, J. Y., Tsai, M. H., Kanno, S., Lung, H. F., & Liu, T. Y. (2024). Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis. bioRxiv, 2024-06.