Minute™ Synaptosome Isolation Kit (50 preps)

Manual & Protocol | Material Safety Data Sheets (MSDS)

The ability to isolate synaptosomes from neuronal tissues/cultured cells is essential for understanding the mechanisms of neurological diseases. Contained in synaptosomes are synaptic vesicles with diameters of 80-200 nm that play an important role in signal transmission. Traditionally, synaptosomes are isolated by density ultracentrifugation. The procedure is relatively tedious and time-consuming. A larger amount of starting material is usually required. Minute^TM synaptosome isolation kit provides a simple and rapid method for isolating synaptosomes from fresh/frozen neuronal tissues/cultured cells. The protocol can be completed in less than 1 h without using Dounce homogenizer and ultracentrifugation. The amount of starting material required (10-50 mg tissue) is only a fraction of that required by the traditional method. The buffers used are detergent-free and synaptosome associated proteins are isolated in native form.

Fig.1. Isolation of Synaptosomes from Fresh Mouse Cortex.

A, Ponceau S-stained blot. B, Western blot. Lane 1. Total tissue lysate;

Lane 2. Cytosolic fraction; Lane 3. Synaptosome fraction. Synaptosome

marker antibodies (GluN2B and Glu A2/A3/A4) are from Cell Signaling.

Kit Component:

1.  Gu, J., Ke, P., Guo, H., Liu, J., Liu, Y., Tian, X., ... & Xiao, F. (2023). KCTD13-mediated ubiquitination and degradation of GluN1 regulates excitatory synaptic transmission and seizure susceptibility. Cell Death & Differentiation, 1-16.

2. Ke, P., Gu, J., Liu, J., Liu, Y., Tian, X., Ma, Y., ... & Xiao, F. (2023). Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B. Cell Death Discovery, 9(1), 187.
3. Han, X., Huang, S., Zhuang, Z., Zhang, X., Xie, M., Lou, N., ... & Chen, S. (2023). Phosphatidate phosphatase Lipin1 involves in diabetic encephalopathy pathogenesis via regulating synaptic mitochondrial dynamics. Redox Biology, 102996.